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Hyaluronan-Based Nanohydrogels for Targeting Intracellular S. Aureus in Human Keratinocytes

Montanari, Elita; Oates, Angela; Di Meo, Chiara; Meade, Josephine; Cerrone, Rugiada; Francioso, Antonio; Devine, Deirdre; Coviello, Tommasina; Mancini, Patrizia; Mosca, Luciana; Matricardi, Pietro


Elita Montanari

Chiara Di Meo

Josephine Meade

Rugiada Cerrone

Antonio Francioso

Deirdre Devine

Tommasina Coviello

Patrizia Mancini

Luciana Mosca

Pietro Matricardi


© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Staphylococcus aureus is one of the most significant human pathogens that is frequently isolated in a wide range of superficial and systemic infections. The ability of S. aureus to invade and survive within host cells such as keratinocytes and host immune cells has been increasingly recognized as a potential factor in persistent infections and treatment failures. The incorporation of antibiotics into hyaluronan-cholesterol nanohydrogels represents a novel paradigm in the delivery of therapeutic agents against intracellular bacteria. The work presented herein shows that NHs quickly enter human keratinocytes and accumulate into lysosomes. When used for targeting intracellular S. aureus the antimicrobial activity of loaded levofloxacin is enhanced, possibly changing the antibiotic intracellular fate from cytosol to lysosome. Indeed, gentamicin, an antibiotic that predominantly accumulates in lysosomes, shows significant and equal antibacterial activity when entrapped into NHs. These results strongly suggest that lysosomal formulations may display preferential activity toward intracellular S. aureus, opening new avenues for the use of HA-based NHs for treatment of such skin infections.


Montanari, E., Oates, A., Di Meo, C., Meade, J., Cerrone, R., Francioso, A., …Matricardi, P. (2018). Hyaluronan-Based Nanohydrogels for Targeting Intracellular S. Aureus in Human Keratinocytes. Advanced Healthcare Materials, 7(12), Article 1701483.

Journal Article Type Article
Acceptance Date Mar 16, 2018
Online Publication Date Apr 25, 2018
Publication Date Jun 20, 2018
Deposit Date Jan 16, 2019
Publicly Available Date Oct 27, 2022
Journal Advanced Healthcare Materials
Print ISSN 2192-2640
Electronic ISSN 2192-2659
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 7
Issue 12
Article Number 1701483
Keywords Pharmaceutical Science; Biomaterials; Biomedical Engineering
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