The toxic effect of cytostatics on primary cilia frequency and multiciliation

Filipová, Alžběta; Diaz Garcia, Daniel; Bezrouk, Aleš; Čížková, Dana; Dvořák, Josef; Filip, Stanislav; Sturge, Justin; Šinkorová, Zuzana

Abstract

The primary cilium is considered as a key component of morphological cellular stability. However, cancer cells are notorious for lacking primary cilia in most cases, depending upon the tumour type. Previous reports have shown the effect of starvation and cytostatics on ciliogenesis in normal and cancer cells although with limited success, especially when concerning the latter. In this study we evaluated the presence and frequency of primary cilia in breast fibroblasts and in triple negative breast cancer cells after treatment with cytostatics finding that, in the case of breast fibroblasts, primary cilia were detected at their highest incidence 72 hours after treatment with 120 nM doxorubicin. Further, multiciliated cells were also detected after treatment with 80 nM doxorubicin. On the other hand, treatment with taxol increased the number of ciliated cells only at low concentrations (1.25 and 3.25 nM) and did not induce multiciliation. Interestingly, triple negative breast cancer cells did not present primary cilia after treatment with either doxorubicin or taxol. This is the first study reporting presence of multiple primary cilia in breast fibroblasts induced by Doxorubicin. However, the null effect of these cytostatics on primary cilia incidence in the evaluated TNBC cell lines requires further research.

Citation

Filipová, A., Diaz Garcia, D., Bezrouk, A., Čížková, D., Dvořák, J., Filip, S., …Šinkorová, Z. (2019). The toxic effect of cytostatics on primary cilia frequency and multiciliation. Journal of Cellular and Molecular Medicine, 23(8), 5728-5736. https://doi.org/10.1111/jcmm.14487

• Journal Article Type: Article
• Acceptance Date: May 20, 2019
• Online Publication Date: Jun 17, 2019
• Publication Date: 2019-08
• Deposit Date: May 21, 2019
• Publicly Available Date: Jul 18, 2019
• Journal: Journal of Cellular and Molecular Medicine
• Print ISSN: 1582-1838
• Publisher: Wiley Open Access
• Peer Reviewed: Peer Reviewed
• Volume: 23
• Issue: 8
• Pages: 5728-5736
• Series ISSN: 1582-4934
• DOI: https://doi.org/10.1111/jcmm.14487
• Keywords: Primary cilium; Multiple cilia; Cytostatics; Toxicity; Cell line
• Public URL: https://hull-repository.worktribe.com/output/1821597
• Publisher URL: https://onlinelibrary.wiley.com/doi/full/10.1111/jcmm.14487