A 18F radiolabelled Zn(ii) sensing fluorescent probe

Price, Thomas W.; Firth, George; Eling, Charlotte J.; Kinnon, Michelle; Long, Nicholas J.; Sturge, Justin; Stasiuk, Graeme J.

Abstract

A selective fluorescent probe for Zn(ii), AQA-F, has been synthesized. AQA-F exhibits a ratiometric shift in emission of up to 80 nm upon binding Zn(ii) ([AQA-F] = 0.1 mM, [Zn(ii)Cl 2 ] = 0-300 μM). An enhancement of quantum yield from Φ = 4.2% to Φ = 35% is also observed. AQA-F has a binding constant, K d = 15.2 μM with Zn(ii). This probe has been shown to respond to endogenous Zn(ii) levels in vitro in prostate and prostate cancer cell lines. [ 18 F]AQA-F has been synthesized with a radiochemical yield of 8.6% and a radiochemical purity of 97% in 88 minutes. AQA-F shows the potential for a dual modal PET/fluorescence imaging probe for Zn(ii).

Citation

Price, T. W., Firth, G., Eling, C. J., Kinnon, M., Long, N. J., Sturge, J., & Stasiuk, G. J. (2018). A 18F radiolabelled Zn(ii) sensing fluorescent probe. Chemical communications : Chem comm / the Royal Society of Chemistry, 54(26), 3227-3230. https://doi.org/10.1039/c8cc00687c

• Journal Article Type: Article
• Acceptance Date: Mar 5, 2018
• Online Publication Date: Mar 5, 2018
• Publication Date: Mar 12, 2018
• Deposit Date: Aug 2, 2018
• Publicly Available Date: Mar 6, 2019
• Journal: Chemical Communications
• Print ISSN: 1359-7345
• Electronic ISSN: 1364-548X
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 54
• Issue: 26
• Pages: 3227-3230
• DOI: https://doi.org/10.1039/c8cc00687c
• Public URL: https://hull-repository.worktribe.com/output/774341
• Publisher URL: http://pubs.rsc.org/en/Content/ArticleLanding/2018/CC/C8CC00687C#!divAbstract