A 18F radiolabelled Zn(ii) sensing fluorescent probe

Price, Thomas W..; Firth, George.; Eling, Charlotte J..; Kinnon, Michelle.; Long, Nicholas J..; Sturge, Justin.; Stasiuk, Graeme J..

doi:10.1039/c8cc00687c

Authors

Thomas W. Price

George Firth

Charlotte J. Eling

Michelle Kinnon

Nicholas J. Long

Dr Justin Sturge J.Sturge@hull.ac.uk
Reader

Dr Graeme Stasiuk G.Stasiuk@hull.ac.uk
Non-Clinical Lecturer in Molecular Imaging

Abstract

A selective fluorescent probe for Zn(ii), AQA-F, has been synthesized. AQA-F exhibits a ratiometric shift in emission of up to 80 nm upon binding Zn(ii) ([AQA-F] = 0.1 mM, [Zn(ii)Cl 2 ] = 0-300 μM). An enhancement of quantum yield from Φ = 4.2% to Φ = 35% is also observed. AQA-F has a binding constant, K d = 15.2 μM with Zn(ii). This probe has been shown to respond to endogenous Zn(ii) levels in vitro in prostate and prostate cancer cell lines. [ 18 F]AQA-F has been synthesized with a radiochemical yield of 8.6% and a radiochemical purity of 97% in 88 minutes. AQA-F shows the potential for a dual modal PET/fluorescence imaging probe for Zn(ii).

Journal Article Type	Article
Publication Date	Mar 12, 2018
Journal	Chemical Communications
Print ISSN	1359-7345
Electronic ISSN	1364-548X
Publisher	Royal Society of Chemistry
Peer Reviewed	Peer Reviewed
Volume	54
Issue	26
Pages	3227-3230
Institution Citation	Price, T. W., Firth, G., Eling, C. J., Kinnon, M., Long, N. J., Sturge, J., & Stasiuk, G. J. (2018). A 18F radiolabelled Zn(ii) sensing fluorescent probe. Chemical communications : Chem comm / the Royal Society of Chemistry, 54(26), 3227-3230. https://doi.org/10.1039/c8cc00687c
DOI	https://doi.org/10.1039/c8cc00687c
Publisher URL	http://pubs.rsc.org/en/Content/ArticleLanding/2018/CC/C8CC00687C#!divAbstract
Copyright Statement	©2018 The authors