Effect of Rocket (Eruca sativa) Extract on MRSA Growth and Proteome: Metabolic Adjustments in Plant-Based Media

Doulgeraki, Agapi I.; Efthimiou, Georgios; Paramithiotis, Spiros; Pappas, Katherine M.; Typas, Milton A.; Nychas, George-John

doi:10.3389/fmicb.2017.00782

Authors

Agapi I. Doulgeraki

Dr Georgios Efthimiou G.Efthimiou@hull.ac.uk
Lecturer in Microbiology

Spiros Paramithiotis

Katherine M. Pappas

Milton A. Typas

George-John Nychas

Abstract

The emergence of methicillin-resistant Staphylococcus aureus (MRSA) in food has provoked a great concern about the presence of MRSA in associated foodstuff. Although MRSA is often detected in various retailed meat products, it seems that food handlers are more strongly associated with this type of food contamination. Thus, it can be easily postulated that any food could be contaminated with this pathogen in an industrial environment or in household and cause food poisoning. To this direction, the effect of rocket (Eruca sativa) extract on MRSA growth and proteome was examined in the present study. This goal was achieved with the comparative study of the MRSA strain COL proteome, cultivated in rocket extract versus the standard Luria-Bertani growth medium. The obtained results showed that MRSA was able to grow in rocket extract. In addition, proteome analysis using 2-DE method showed that MRSA strain COL is taking advantage of the sugar-, lipid-, and vitamin-rich substrate in the liquid rocket extract, although its growth was delayed in rocket extract compared to Luria–Bertani medium. This work could initiate further research about bacterial metabolism in plant-based media and defense mechanisms against plant-derived antibacterials.

Citation

Doulgeraki, A. I., Efthimiou, G., Paramithiotis, S., Pappas, K. M., Typas, M. A., & Nychas, G. (2017). Effect of Rocket (Eruca sativa) Extract on MRSA Growth and Proteome: Metabolic Adjustments in Plant-Based Media. Frontiers in Microbiology, 8, https://doi.org/10.3389/fmicb.2017.00782

Journal Article Type	Article
Acceptance Date	Apr 18, 2017
Online Publication Date	May 5, 2017
Publication Date	May 5, 2017
Deposit Date	Dec 18, 2019
Journal	Frontiers in Microbiology
Print ISSN	1664-302X
Publisher	Frontiers Media
Peer Reviewed	Peer Reviewed
Volume	8
Article Number	782
DOI	https://doi.org/10.3389/fmicb.2017.00782
Keywords	MRSA; Proteome; Metabolism; Defense; Extract; Rocket
Public URL	https://hull-repository.worktribe.com/output/2037885
Publisher URL	https://www.frontiersin.org/articles/10.3389/fmicb.2017.00782/full