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Effect of Rocket (Eruca sativa) Extract on MRSA Growth and Proteome: Metabolic Adjustments in Plant-Based Media

Doulgeraki, Agapi I.; Efthimiou, Georgios; Paramithiotis, Spiros; Pappas, Katherine M.; Typas, Milton A.; Nychas, George-John

Authors

Agapi I. Doulgeraki

Spiros Paramithiotis

Katherine M. Pappas

Milton A. Typas

George-John Nychas



Abstract

The emergence of methicillin-resistant Staphylococcus aureus (MRSA) in food has provoked a great concern about the presence of MRSA in associated foodstuff. Although MRSA is often detected in various retailed meat products, it seems that food handlers are more strongly associated with this type of food contamination. Thus, it can be easily postulated that any food could be contaminated with this pathogen in an industrial environment or in household and cause food poisoning. To this direction, the effect of rocket (Eruca sativa) extract on MRSA growth and proteome was examined in the present study. This goal was achieved with the comparative study of the MRSA strain COL proteome, cultivated in rocket extract versus the standard Luria-Bertani growth medium. The obtained results showed that MRSA was able to grow in rocket extract. In addition, proteome analysis using 2-DE method showed that MRSA strain COL is taking advantage of the sugar-, lipid-, and vitamin-rich substrate in the liquid rocket extract, although its growth was delayed in rocket extract compared to Luria–Bertani medium. This work could initiate further research about bacterial metabolism in plant-based media and defense mechanisms against plant-derived antibacterials.

Citation

Doulgeraki, A. I., Efthimiou, G., Paramithiotis, S., Pappas, K. M., Typas, M. A., & Nychas, G. (2017). Effect of Rocket (Eruca sativa) Extract on MRSA Growth and Proteome: Metabolic Adjustments in Plant-Based Media. Frontiers in Microbiology, 8, https://doi.org/10.3389/fmicb.2017.00782

Journal Article Type Article
Acceptance Date Apr 18, 2017
Online Publication Date May 5, 2017
Publication Date May 5, 2017
Deposit Date Dec 18, 2019
Journal Frontiers in Microbiology
Print ISSN 1664-302X
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 8
Article Number 782
DOI https://doi.org/10.3389/fmicb.2017.00782
Keywords MRSA; Proteome; Metabolism; Defense; Extract; Rocket
Public URL https://hull-repository.worktribe.com/output/2037885
Publisher URL https://www.frontiersin.org/articles/10.3389/fmicb.2017.00782/full

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