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Controlling the antimicrobial action of surface modified magnesium hydroxide nanoparticles

Halbus, Ahmed F.; Horozov, Tommy S.; Paunov, Vesselin N.


Ahmed F. Halbus

Vesselin N. Paunov


Ahmed F. Halbus

Vesselin N. Paunov
Project Leader


Magnesium hydroxide nanoparticles (Mg(OH)2NPs) have recently attracted significant attention due to their wide applications as environmentally friendly antimicrobial nanomaterials, with potentially low toxicity and low fabrication cost. Here, we describe the synthesis and characterisation of a range of surface modified Mg(OH)2NPs, including particle size distribution, crystallite size, zeta potential, isoelectric point, X-ray diffraction (XRD), dynamic light scattering (DLS), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), energy dispersive X-ray analysis (EDX), Fourier transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM). We explored the antimicrobial activity of the modified Mg(OH)2NPs on the microalgae (C. reinhardtii), yeast (S. cerevisiae) and Escherichia coli (E. coli). The viability of these cells was evaluated for various concentrations and exposure times with Mg(OH)2NPs. It was discovered that the antimicrobial activity of the uncoated Mg(OH)2NPs on the viability of C. reinhardtii occurred at considerably lower particle concentrations than for S. cerevisiae and E. coli. Our results indicate that the antimicrobial activity of polyelectrolyte-coated Mg(OH)2NPs alternates with their surface charge. The anionic nanoparticles (Mg(OH)2NPs/PSS) have much lower antibacterial activity than the cationic ones (Mg(OH)2NPs/PSS/PAH and uncoated Mg(OH)2NPs). These findings could be explained by the lower adhesion of the Mg(OH)2NPs/PSS to the cell wall, because of electrostatic repulsion and the enhanced particle-cell adhesion due to electrostatic attraction in the case of cationic Mg(OH)2NPs. The results can be potentially applied to control the cytotoxicity and the antimicrobial activity of other inorganic nanoparticles.


Halbus, A. F., Horozov, T. S., & Paunov, V. N. (2019). Controlling the antimicrobial action of surface modified magnesium hydroxide nanoparticles. Biomimetics, 4(2), Article 41.

Journal Article Type Article
Acceptance Date Jun 20, 2019
Online Publication Date Jun 25, 2019
Publication Date 2019-06
Deposit Date Jun 28, 2019
Publicly Available Date Jun 28, 2019
Journal Biomimetics
Electronic ISSN 2313-7673
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 4
Issue 2
Article Number 41
Keywords Mg(OH)2NPs; magnesium hydroxide; polyelectrolytes; poly (styrene sulfonate); poly (allyl amine) hydrochloride; antimicrobial nanoparticles; algae; yeast; bacteria; C. reinhardtii; S.cerevisiae; E. coli
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Copyright Statement
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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