David R. J. Riley
Coronin 1 Is Required for Integrin β2 Translocation in Platelets
Riley, David R. J.; Khalil, Jawad S.; Pieters, Jean; Naseem, Khalid M.; Rivero, Francisco
Authors
Jawad S. Khalil
Jean Pieters
Khalid M. Naseem
Dr Francisco Rivero Crespo F.Rivero-Crespo@hull.ac.uk
Reader in Biomedical Science
Abstract
Remodeling of the actin cytoskeleton is one of the critical events that allows platelets to undergo morphological and functional changes in response to receptor-mediated signaling cascades. Coronins are a family of evolutionarily conserved proteins implicated in the regulation of the actin cytoskeleton, represented by the abundant coronins 1, 2, and 3 and the less abundant coronin 7 in platelets, but their functions in these cells are poorly understood. A recent report revealed impaired agonist-induced actin polymerization and cofilin phosphoregulation and altered thrombus formation in vivo as salient phenotypes in the absence of an overt hemostasis defect in vivo in a knockout mouse model of coronin 1. Here we show that the absence of coronin 1 is associated with impaired translocation of integrin β2 to the platelet surface upon stimulation with thrombin while morphological and functional alterations, including defects in Arp2/3 complex localization and cAMP-dependent signaling, are absent. Our results suggest a large extent of functional overlap among coronins 1, 2, and 3 in platelets, while aspects like integrin β2 translocation are specifically or predominantly dependent on coronin 1.
Citation
Riley, D. R. J., Khalil, J. S., Pieters, J., Naseem, K. M., & Rivero, F. (in press). Coronin 1 Is Required for Integrin β2 Translocation in Platelets. International Journal of Molecular Sciences, 21(1), Article 356. https://doi.org/10.3390/ijms21010356
Journal Article Type | Article |
---|---|
Acceptance Date | Jan 1, 2020 |
Online Publication Date | Jan 5, 2020 |
Deposit Date | Jan 11, 2020 |
Publicly Available Date | Jan 13, 2020 |
Journal | International Journal of Molecular Sciences |
Publisher | MDPI |
Peer Reviewed | Peer Reviewed |
Volume | 21 |
Issue | 1 |
Article Number | 356 |
DOI | https://doi.org/10.3390/ijms21010356 |
Keywords | Physical and Theoretical Chemistry; Inorganic Chemistry; Organic Chemistry; Spectroscopy; Molecular Biology; Catalysis; General Medicine; Computer Science Applications; actin; Arp2/3 complex; cAMP; coronin 1; integrin β2; platelets; thrombin; collagen; p |
Public URL | https://hull-repository.worktribe.com/output/3353959 |
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