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Coronin 1 Is Required for Integrin β2 Translocation in Platelets

Riley, David R. J.; Khalil, Jawad S.; Pieters, Jean; Naseem, Khalid M.; Rivero, Francisco

Authors

David R. J. Riley

Jawad S. Khalil

Jean Pieters

Khalid M. Naseem

Francisco Rivero



Abstract

Remodeling of the actin cytoskeleton is one of the critical events that allows platelets to undergo morphological and functional changes in response to receptor-mediated signaling cascades. Coronins are a family of evolutionarily conserved proteins implicated in the regulation of the actin cytoskeleton, represented by the abundant coronins 1, 2, and 3 and the less abundant coronin 7 in platelets, but their functions in these cells are poorly understood. A recent report revealed impaired agonist-induced actin polymerization and cofilin phosphoregulation and altered thrombus formation in vivo as salient phenotypes in the absence of an overt hemostasis defect in vivo in a knockout mouse model of coronin 1. Here we show that the absence of coronin 1 is associated with impaired translocation of integrin β2 to the platelet surface upon stimulation with thrombin while morphological and functional alterations, including defects in Arp2/3 complex localization and cAMP-dependent signaling, are absent. Our results suggest a large extent of functional overlap among coronins 1, 2, and 3 in platelets, while aspects like integrin β2 translocation are specifically or predominantly dependent on coronin 1.

Journal Article Type Article
Journal International Journal of Molecular Sciences
Print ISSN 1661-6596
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 21
Issue 1
Article Number 356
APA6 Citation Riley, D. R. J., Khalil, J. S., Pieters, J., Naseem, K. M., & Rivero, F. (in press). Coronin 1 Is Required for Integrin β2 Translocation in Platelets. International Journal of Molecular Sciences, 21(1), https://doi.org/10.3390/ijms21010356
DOI https://doi.org/10.3390/ijms21010356
Keywords Physical and Theoretical Chemistry; Inorganic Chemistry; Organic Chemistry; Spectroscopy; Molecular Biology; Catalysis; General Medicine; Computer Science Applications; actin; Arp2/3 complex; cAMP; coronin 1; integrin β2; platelets; thrombin; collagen; pr

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