Coronin 1 Is Required for Integrin β2 Translocation in Platelets

Riley, David R. J.; Khalil, Jawad S.; Pieters, Jean; Naseem, Khalid M.; Rivero, Francisco

Abstract

Remodeling of the actin cytoskeleton is one of the critical events that allows platelets to undergo morphological and functional changes in response to receptor-mediated signaling cascades. Coronins are a family of evolutionarily conserved proteins implicated in the regulation of the actin cytoskeleton, represented by the abundant coronins 1, 2, and 3 and the less abundant coronin 7 in platelets, but their functions in these cells are poorly understood. A recent report revealed impaired agonist-induced actin polymerization and cofilin phosphoregulation and altered thrombus formation in vivo as salient phenotypes in the absence of an overt hemostasis defect in vivo in a knockout mouse model of coronin 1. Here we show that the absence of coronin 1 is associated with impaired translocation of integrin β2 to the platelet surface upon stimulation with thrombin while morphological and functional alterations, including defects in Arp2/3 complex localization and cAMP-dependent signaling, are absent. Our results suggest a large extent of functional overlap among coronins 1, 2, and 3 in platelets, while aspects like integrin β2 translocation are specifically or predominantly dependent on coronin 1.

Citation

Riley, D. R. J., Khalil, J. S., Pieters, J., Naseem, K. M., & Rivero, F. (in press). Coronin 1 Is Required for Integrin β2 Translocation in Platelets. International Journal of Molecular Sciences, 21(1), Article 356. https://doi.org/10.3390/ijms21010356

• Journal Article Type: Article
• Acceptance Date: Jan 1, 2020
• Online Publication Date: Jan 5, 2020
• Deposit Date: Jan 11, 2020
• Publicly Available Date: Jan 13, 2020
• Journal: International Journal of Molecular Sciences
• Electronic ISSN: 1422-0067
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 21
• Issue: 1
• Article Number: 356
• DOI: https://doi.org/10.3390/ijms21010356
• Keywords: Physical and Theoretical Chemistry; Inorganic Chemistry; Organic Chemistry; Spectroscopy; Molecular Biology; Catalysis; General Medicine; Computer Science Applications; actin; Arp2/3 complex; cAMP; coronin 1; integrin β2; platelets; thrombin; collagen; p
• Public URL: https://hull-repository.worktribe.com/output/3353959
• Contract Date: Jan 13, 2020