Effect of induced hypoglycemia on inflammation and oxidative stress in type 2 diabetes and control subjects
Kahal, Hassan; Halama, Anna; Aburima, Ahmed; Bhagwat, Aditya M.; Butler, Alexandra E.; Grauman, Johannes; Suhre, Karsten; Sathyapalan, Thozhukat; Atkin, Stephen L.
Aditya M. Bhagwat
Alexandra E. Butler
Stephen L. Atkin
Intensive diabetes control has been associated with increased mortality in type 2 diabetes (T2DM); this has been suggested to be due to increased hypoglycemia. We measured hypoglycemia-induced changes in endothelial parameters, oxidative stress markers and inflammation at baseline and after a 24-hour period in type 2 diabetic (T2DM) subjects versus age-matched controls. Case-control study: 10 T2DM and 8 control subjects. Blood glucose was reduced from 5 (90 mg/dl) to hypoglycemic levels of 2.8 mmol/L (50 mg/dl) for 1 hour by incremental hyperinsulinemic clamps using baseline and 24 hour samples. Measures of endothelial parameters, oxidative stress and inflammation at baseline and at 24-hours post hypoglycemia were performed: proteomic (Somalogic) analysis for inflammatory markers complemented by C-reactive protein (hsCRP) measurement, and proteomic markers and urinary isoprostanes for oxidative measures, together with endothelial function. Between baseline and 24 -hours after hypoglycemia, 15 of 140 inflammatory proteins differed in T2DM whilst only 1 of 140 differed in controls; all returned to baseline at 24-hours. However, elevated hsCRP levels were seen at 24-hours in T2DM (2.4 mg/L (1.2–5.4) vs. 3.9 mg/L (1.8–6.1), Baseline vs 24-hours, P
|Journal Article Type||Article|
|Publisher||Nature Publishing Group|
|Peer Reviewed||Peer Reviewed|
|APA6 Citation||Kahal, H., Halama, A., Aburima, A., Bhagwat, A. M., Butler, A. E., Grauman, J., …Atkin, S. L. (2020). Effect of induced hypoglycemia on inflammation and oxidative stress in type 2 diabetes and control subjects. Scientific reports, 10(1), https://doi.org/10.1038/s41598-020-61531-z|
|Keywords||Diabetes complications; Type 2 diabetes|
|Additional Information||Received: 20 November 2019; Accepted: 28 February 2020; First Online: 16 March 2020; : The authors declare no competing interests.|
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