Hassan Kahal
Effect of induced hypoglycemia on inflammation and oxidative stress in type 2 diabetes and control subjects
Kahal, Hassan; Halama, Anna; Aburima, Ahmed; Bhagwat, Aditya M.; Butler, Alexandra E.; Grauman, Johannes; Suhre, Karsten; Sathyapalan, Thozhukat; Atkin, Stephen L.
Authors
Anna Halama
Dr Ahmed Aburima A.Aburima@hull.ac.uk
Lecturer in Cardiovascular Science
Aditya M. Bhagwat
Alexandra E. Butler
Johannes Grauman
Karsten Suhre
Professor Thozhukat Sathyapalan T.Sathyapalan@hull.ac.uk
Professor of Diabetes, Endocrinology and Metabolism
Stephen L. Atkin
Abstract
Intensive diabetes control has been associated with increased mortality in type 2 diabetes (T2DM); this has been suggested to be due to increased hypoglycemia. We measured hypoglycemia-induced changes in endothelial parameters, oxidative stress markers and inflammation at baseline and after a 24-hour period in type 2 diabetic (T2DM) subjects versus age-matched controls. Case-control study: 10 T2DM and 8 control subjects. Blood glucose was reduced from 5 (90 mg/dl) to hypoglycemic levels of 2.8 mmol/L (50 mg/dl) for 1 hour by incremental hyperinsulinemic clamps using baseline and 24 hour samples. Measures of endothelial parameters, oxidative stress and inflammation at baseline and at 24-hours post hypoglycemia were performed: proteomic (Somalogic) analysis for inflammatory markers complemented by C-reactive protein (hsCRP) measurement, and proteomic markers and urinary isoprostanes for oxidative measures, together with endothelial function. Between baseline and 24 -hours after hypoglycemia, 15 of 140 inflammatory proteins differed in T2DM whilst only 1 of 140 differed in controls; all returned to baseline at 24-hours. However, elevated hsCRP levels were seen at 24-hours in T2DM (2.4 mg/L (1.2–5.4) vs. 3.9 mg/L (1.8–6.1), Baseline vs 24-hours, P < 0.05). In patients with T2DM, between baseline and 24-hour after hypoglycemia, only one of 15 oxidative stress proteins differed and this was not seen in controls. An increase (P = 0.016) from baseline (73.4 ng/mL) to 24 hours after hypoglycemia (91.7 ng/mL) was seen for urinary isoprostanes. Hypoglycemia resulted in inflammatory and oxidative stress markers being elevated in T2DM subjects but not controls 24-hours after the event.
Citation
Kahal, H., Halama, A., Aburima, A., Bhagwat, A. M., Butler, A. E., Grauman, J., Suhre, K., Sathyapalan, T., & Atkin, S. L. (2020). Effect of induced hypoglycemia on inflammation and oxidative stress in type 2 diabetes and control subjects. Scientific reports, 10(1), Article 4750. https://doi.org/10.1038/s41598-020-61531-z
Journal Article Type | Article |
---|---|
Acceptance Date | Feb 28, 2020 |
Online Publication Date | Mar 16, 2020 |
Publication Date | 2020-12 |
Deposit Date | Mar 24, 2020 |
Publicly Available Date | Mar 25, 2020 |
Journal | Scientific Reports |
Print ISSN | 2045-2322 |
Publisher | Nature Publishing Group |
Peer Reviewed | Peer Reviewed |
Volume | 10 |
Issue | 1 |
Article Number | 4750 |
DOI | https://doi.org/10.1038/s41598-020-61531-z |
Keywords | Diabetes complications; Type 2 diabetes |
Public URL | https://hull-repository.worktribe.com/output/3481139 |
Publisher URL | https://www.nature.com/articles/s41598-020-61531-z#article-info |
Additional Information | Received: 20 November 2019; Accepted: 28 February 2020; First Online: 16 March 2020; : The authors declare no competing interests. |
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