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Platelet function following induced hypoglycaemia in type 2 diabetes

Kahal, H.; Aburima, A.; Spurgeon, B.; Wraith, K. S.; Rigby, A. S.; Sathyapalan, T.; Kilpatrick, E. S.; Naseem, K. M.; Atkin, S. L.

Authors

H. Kahal

A. Aburima

B. Spurgeon

K. S. Wraith

A. S. Rigby

T. Sathyapalan

E. S. Kilpatrick

K. M. Naseem

S. L. Atkin



Abstract

Aim
Strict glycaemic control has been associated with an increased mortality rate in subjects with type 2 diabetes (T2DM). Here we examined platelet function immediately and 24 hours following induced hypoglycaemia in people with type 2 diabetes compared to healthy age-matched controls.

Methods
Hyperinsulinaemic clamps reduced blood glucose to 2.8 mmol/L (50 mg/dl) for 1 hour. Sampling at baseline; euglycaemia 5mmol/L (90 mg/dl); hypoglycaemia; and at 24 post clamp were undertaken. Platelet function was measured by whole blood flow cytometry.

Results
10 subjects with T2DM and 8 controls were recruited. Platelets from people with T2DM showed reduced sensitivity to prostacyclin (PGI2, 1 nM) following hypoglycaemia. The ability of PGI2 to inhibit platelet activation was significantly impaired at 24 hours compared to baseline in the T2DM group. Here, inhibition of fibrinogen binding was 29.5% (10.3 – 43.8) compared to 50.8% (36.8 – 61.1), (P < 0.05), while inhibition of P-selectin expression was 32% (16.1 – 47.6) vs. 54.4% (42.5 – 67.5) (P < 0.05). No significant changes in platelet function were noted in controls.

Conclusion
Induced hypoglycaemia in T2DM enhances platelet hyperactivity through impaired sensitivity to prostacyclin at 24 hours.

Citation

Kahal, H., Aburima, A., Spurgeon, B., Wraith, K. S., Rigby, A. S., Sathyapalan, T., …Atkin, S. L. (2018). Platelet function following induced hypoglycaemia in type 2 diabetes. Diabetes & metabolism, 44(5), 431-436. https://doi.org/10.1016/j.diabet.2018.04.004

Journal Article Type Article
Acceptance Date Apr 18, 2018
Online Publication Date May 3, 2018
Publication Date Nov 1, 2018
Deposit Date May 6, 2018
Publicly Available Date May 4, 2019
Journal Diabetes & Metabolism
Print ISSN 1262-3636
Electronic ISSN 1878-1780
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 44
Issue 5
Pages 431-436
DOI https://doi.org/10.1016/j.diabet.2018.04.004
Keywords Internal Medicine; Endocrinology, Diabetes and Metabolism; Endocrinology; General Medicine
Public URL https://hull-repository.worktribe.com/output/821934
Publisher URL https://www.sciencedirect.com/science/article/pii/S1262363618300818
Copyright Statement © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/

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