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An optical sensor for reactive oxygen species: encapsulation of functionalised silica nanoparticles into silicate nanoprobes to reduce fluorophore leaching

Hammond, Victoria J.; Aylott, Jonathan W.; Greenway, Gillian M.; Watts, Paul; Webster, Abigail; Wiles, Charlotte

Authors

Victoria J. Hammond

Jonathan W. Aylott

Gillian M. Greenway G.M.Greenway@hull.ac.uk

Paul Watts

Abigail Webster

Charlotte Wiles



Abstract

Sol-gel nanoprobes, also known as Photonic Explorer for Bioanalysis with Biologically Localised Embedding (PEBBLE), capable of performing in-vitro intracellular monitoring of reactive oxygen species have been developed using a modified form of 5(6)-carboxyfluorescein diacetate. A sol-gel matrix was selected for the design of the probes as it is photostable, optically transparent and chemically inert, and to minimise leaching of the dye from the porous matrix it was covalently immobilised to silica nanoparticles (15 nm). Using this approach, 0.1% of the dye was found to leach over a typical analysis time of 5 h and minimal photobleaching was observed. In addition, the nanoprobes were shown to respond to hydrogen peroxide, hydroxyl anions, nitric oxide, peroxynitrile and superoxide anions, obtaining limits of detection of 2.2, 1.1, 3.2, 1.1 and 1.1 nM respectively. The nanoprobes were subsequently introduced into bovine oviducts using a lipid transfection reagent (Escort IV (TM)) and fluorescence was observed.

Journal Article Type Article
Publication Date Jan 1, 2008
Journal Analyst
Print ISSN 0003-2654
Electronic ISSN 1364-5528
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 133
Issue 1
Pages 71-75
APA6 Citation Hammond, V. J., Aylott, J. W., Greenway, G. M., Watts, P., Webster, A., & Wiles, C. (2008). An optical sensor for reactive oxygen species: encapsulation of functionalised silica nanoparticles into silicate nanoprobes to reduce fluorophore leaching. The Analyst, 133(1), 71-75. https://doi.org/10.1039/b711995j
DOI https://doi.org/10.1039/b711995j
Keywords Analytical Chemistry; Spectroscopy; Electrochemistry; Biochemistry; Environmental Chemistry
Publisher URL https://pubs.rsc.org/en/Content/ArticleLanding/2008/AN/B711995J#!divAbstract
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