Enzymatic synthesis of a series of alkyl esters using novozyme 435 in a packed-bed, miniaturized, continuous flow reactor

Woodcock, L. L.; Wiles, C.; Greenway, G. M.; Watts, P.; Wells, A.; Eyley, S.

Abstract

Using lipase catalysed, enzymatic esterification as a model reaction, we successfully demonstrate the use of miniaturized technology for biocatalytic reactions. Benchmarked against batch reactions, nine alkyl esters have been synthesized effectively, using Novozyme 435 in a pressure driven, packed-bed, miniaturized, continuous flow reactor. In some cases close to 100% ester conversions were obtained. The paper also demonstrates the ability to screen the enzyme for substrate specificity.

Citation

Woodcock, L. L., Wiles, C., Greenway, G. M., Watts, P., Wells, A., & Eyley, S. (2008). Enzymatic synthesis of a series of alkyl esters using novozyme 435 in a packed-bed, miniaturized, continuous flow reactor. Biocatalysis and biotransformation, 26(6), 466-472. https://doi.org/10.1080/10242420802456571

• Journal Article Type: Article
• Acceptance Date: Dec 31, 2008
• Online Publication Date: Jul 11, 2009
• Publication Date: 2008-01
• Journal: BIOCATALYSIS AND BIOTRANSFORMATION
• Print ISSN: 1024-2422
• Publisher: Taylor and Francis
• Peer Reviewed: Peer Reviewed
• Volume: 26
• Issue: 6
• Pages: 466-472
• DOI: https://doi.org/10.1080/10242420802456571
• Keywords: Biotechnology; Biochemistry; Catalysis
• Public URL: https://hull-repository.worktribe.com/output/391251
• Publisher URL: https://www.tandfonline.com/doi/full/10.1080/10242420802456571