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Enzymatic synthesis of a series of alkyl esters using novozyme 435 in a packed-bed, miniaturized, continuous flow reactor

Woodcock, L. L.; Wiles, C.; Greenway, G. M.; Watts, P.; Wells, A.; Eyley, S.

Authors

L. L. Woodcock

C. Wiles

G. M. Greenway G.M.Greenway@hull.ac.uk

P. Watts

A. Wells

S. Eyley



Abstract

Using lipase catalysed, enzymatic esterification as a model reaction, we successfully demonstrate the use of miniaturized technology for biocatalytic reactions. Benchmarked against batch reactions, nine alkyl esters have been synthesized effectively, using Novozyme 435 in a pressure driven, packed-bed, miniaturized, continuous flow reactor. In some cases close to 100% ester conversions were obtained. The paper also demonstrates the ability to screen the enzyme for substrate specificity.

Journal Article Type Article
Publication Date 2008-01
Journal BIOCATALYSIS AND BIOTRANSFORMATION
Print ISSN 1024-2422
Electronic ISSN 1029-2446
Publisher Taylor & Francis
Peer Reviewed Peer Reviewed
Volume 26
Issue 6
Pages 466-472
APA6 Citation Woodcock, L. L., Wiles, C., Greenway, G. M., Watts, P., Wells, A., & Eyley, S. (2008). Enzymatic synthesis of a series of alkyl esters using novozyme 435 in a packed-bed, miniaturized, continuous flow reactor. Biocatalysis and biotransformation, 26(6), 466-472. https://doi.org/10.1080/10242420802456571
DOI https://doi.org/10.1080/10242420802456571
Keywords Biotechnology; Biochemistry; Catalysis
Publisher URL https://www.tandfonline.com/doi/full/10.1080/10242420802456571