B. Ngamsom
The development and evaluation of a conducting matrix for the electrochemical regeneration of the immobilised co-factor NAD(H) under continuous flow
Ngamsom, B.; Hickey, A. M.; Greenway, G. M.; Littlechild, J. A.; McCreedy, T.; Watts, P.; Wiles, C.
Authors
A. M. Hickey
G. M. Greenway
J. A. Littlechild
T. McCreedy
P. Watts
C. Wiles
Abstract
Through the preparation of a novel controlled pore glass-poly(pyrrole) material we have developed a conducting support that is not only suitable for the co-immobilisation of enzymes and co-factors, but also enables the facile electrochemical regeneration of the co-factor during a reaction. Employing the selective reduction of (rac)-2-phenylpropionaldehyde to (S)-phenyl-1-propanol as a model, we have demonstrated the successful co-immobilisation of the HLADH enzyme and co-factor NAD(H); with incorporation of the material into a continuous flow reactor facilitating the in situ electrochemical regeneration of NAD(H) for in excess of 100 h. Using this approach we have developed a reagent-less, atom efficient system applicable to the cost-effective, continuous biosynthesis of chiral compounds.
Citation
Ngamsom, B., Hickey, A. M., Greenway, G. M., Littlechild, J. A., McCreedy, T., Watts, P., & Wiles, C. (2010). The development and evaluation of a conducting matrix for the electrochemical regeneration of the immobilised co-factor NAD(H) under continuous flow. Organic & biomolecular chemistry, 8(10), 2419-2424. https://doi.org/10.1039/b924100k
Journal Article Type | Article |
---|---|
Acceptance Date | Mar 5, 2010 |
Online Publication Date | Mar 22, 2010 |
Publication Date | May 21, 2010 |
Journal | ORGANIC & BIOMOLECULAR CHEMISTRY |
Print ISSN | 1477-0520 |
Publisher | Royal Society of Chemistry |
Peer Reviewed | Peer Reviewed |
Volume | 8 |
Issue | 10 |
Pages | 2419-2424 |
DOI | https://doi.org/10.1039/b924100k |
Keywords | Physical and Theoretical Chemistry; Organic Chemistry; Biochemistry |
Public URL | https://hull-repository.worktribe.com/output/387084 |
You might also like
Electro-catalytic reactions
(2009)
Thesis
Pollution reduction with processed waste materials
(2013)
Thesis
Downloadable Citations
About Repository@Hull
Administrator e-mail: repository@hull.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2025
Advanced Search