The development and evaluation of a conducting matrix for the electrochemical regeneration of the immobilised co-factor NAD(H) under continuous flow

Ngamsom, B.; Hickey, A. M.; Greenway, G. M.; Littlechild, J. A.; McCreedy, T.; Watts, P.; Wiles, C.

Abstract

Through the preparation of a novel controlled pore glass-poly(pyrrole) material we have developed a conducting support that is not only suitable for the co-immobilisation of enzymes and co-factors, but also enables the facile electrochemical regeneration of the co-factor during a reaction. Employing the selective reduction of (rac)-2-phenylpropionaldehyde to (S)-phenyl-1-propanol as a model, we have demonstrated the successful co-immobilisation of the HLADH enzyme and co-factor NAD(H); with incorporation of the material into a continuous flow reactor facilitating the in situ electrochemical regeneration of NAD(H) for in excess of 100 h. Using this approach we have developed a reagent-less, atom efficient system applicable to the cost-effective, continuous biosynthesis of chiral compounds.

Citation

Ngamsom, B., Hickey, A. M., Greenway, G. M., Littlechild, J. A., McCreedy, T., Watts, P., & Wiles, C. (2010). The development and evaluation of a conducting matrix for the electrochemical regeneration of the immobilised co-factor NAD(H) under continuous flow. Organic & biomolecular chemistry, 8(10), 2419-2424. https://doi.org/10.1039/b924100k

• Journal Article Type: Article
• Acceptance Date: Mar 5, 2010
• Online Publication Date: Mar 22, 2010
• Publication Date: May 21, 2010
• Journal: ORGANIC & BIOMOLECULAR CHEMISTRY
• Print ISSN: 1477-0520
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 8
• Issue: 10
• Pages: 2419-2424
• DOI: https://doi.org/10.1039/b924100k
• Keywords: Physical and Theoretical Chemistry; Organic Chemistry; Biochemistry
• Public URL: https://hull-repository.worktribe.com/output/387084