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The development and evaluation of a conducting matrix for the electrochemical regeneration of the immobilised co-factor NAD(H) under continuous flow

Ngamsom, B.; Hickey, A. M.; Greenway, G. M.; Littlechild, J. A.; McCreedy, T.; Watts, P.; Wiles, C.

Authors

B. Ngamsom

A. M. Hickey

J. A. Littlechild

T. McCreedy T.McCreedy@hull.ac.uk

P. Watts

C. Wiles

Abstract

Through the preparation of a novel controlled pore glass-poly(pyrrole) material we have developed a conducting support that is not only suitable for the co-immobilisation of enzymes and co-factors, but also enables the facile electrochemical regeneration of the co-factor during a reaction. Employing the selective reduction of (rac)-2-phenylpropionaldehyde to (S)-phenyl-1-propanol as a model, we have demonstrated the successful co-immobilisation of the HLADH enzyme and co-factor NAD(H); with incorporation of the material into a continuous flow reactor facilitating the in situ electrochemical regeneration of NAD(H) for in excess of 100 h. Using this approach we have developed a reagent-less, atom efficient system applicable to the cost-effective, continuous biosynthesis of chiral compounds.

Journal Article Type Article
Publication Date May 21, 2010
Journal ORGANIC & BIOMOLECULAR CHEMISTRY
Print ISSN 1477-0520
Electronic ISSN 1477-0539
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 8
Issue 10
Pages 2419-2424
Institution Citation Ngamsom, B., Hickey, A. M., Greenway, G. M., Littlechild, J. A., McCreedy, T., Watts, P., & Wiles, C. (2010). The development and evaluation of a conducting matrix for the electrochemical regeneration of the immobilised co-factor NAD(H) under continuous flow. Organic & biomolecular chemistry, 8(10), 2419-2424. https://doi.org/10.1039/b924100k
DOI https://doi.org/10.1039/b924100k
Keywords Physical and Theoretical Chemistry; Organic Chemistry; Biochemistry