Development of a high throughput screening tool for biotransformations utilising a thermophilic L-aminoacylase enzyme
Ngamsom, B.; Hickey, A. M.; Greenway, G. M.; Littlechild, J. A.; Watts, P.; Wiles, C.
A. M. Hickey
G. M. Greenway G.M.Greenway@hull.ac.uk
J. A. Littlechild
Micro-reactors containing a monolith-immobilised thermophilic l-aminoacylase, from Thermococcus litoralis, have been developed for use in biotransformation reactions and a study has been carried out to investigate the stereospecificity and stability of the immobilised enzyme. The potential to use the developed micro-reactors as a tool for rapid screening of enzyme specificity was demonstrated, confirming that the l-aminoacylase showed a similar substrate specificity to that previously reported of the free enzyme. From this baseline, the technique was employed as a tool to evaluate potential unreported substrates with N-benzoyl- (l-threonine, l-leucine and l-arginine) and N-acetyl- (d,l-serine, d,l-leucine, l-tyrosine and l-lysine) protecting groups. The order of preferred substrates was found to be Phe > Thr > Leu > Arg for N-benzoyl substrates and Phe ≫ Ser > Leu > Met > Tyr > Trp for N-acetyl substrates. It was found that by using the micro-reactor a significantly smaller quantity of enzyme and substrates was required. It was shown that the micro-reactors were still operational in the presence of selected organic solvents, such as ethanol, methanol, acetone, dimethylformamide (DMF) and dimethylsulfoxide (DMSO). The results indicated that a combination of a small amount of an appropriate solvent (5% DMSO) and a higher reaction temperature could be employed in biotransformations where substrate solubility was an issue. © 2009 Elsevier B.V. All rights reserved.
Ngamsom, B., Hickey, A. M., Greenway, G. M., Littlechild, J. A., Watts, P., & Wiles, C. (2010). Development of a high throughput screening tool for biotransformations utilising a thermophilic L-aminoacylase enzyme. Journal of Molecular Catalysis B: Enzymatic, 63(1-2), 81-86. https://doi.org/10.1016/j.molcatb.2009.12.013
|Journal Article Type||Article|
|Acceptance Date||Dec 14, 2009|
|Online Publication Date||Dec 24, 2009|
|Journal||JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC|
|Peer Reviewed||Peer Reviewed|
|Keywords||Process Chemistry and Technology; Biochemistry; Bioengineering; Catalysis|
This file is under embargo due to copyright reasons.
You might also like
Multiplex sorting of foodborne pathogens by on-chip free-flow magnetophoresis