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An internal sequence targets Trypanosoma brucei triosephosphate isomerase to glycosomes

Galland, Nathalie; de Walque, Stéphane; Voncken, Frank G.J.; Verlinde, Christophe L.M.J.; Michels, Paul A.M.

Authors

Nathalie Galland

Stéphane de Walque

Frank G.J. Voncken F.Voncken@hull.ac.uk

Christophe L.M.J. Verlinde

Paul A.M. Michels



Abstract

In kinetoplastid protists, glycolysis is compartmentalized in glycosomes, organelles belonging to the peroxisome family. The Trypanosoma brucei glycosomal enzyme triosephosphate isomerase (TPI) does not contain either of the two established peroxisome-targeting signals, but we identified a 22 amino acids long fragment, present at an internal position of the polypeptide, that has the capacity to route a reporter protein to glycosomes in transfected trypanosomes, as demonstrated by cell-fractionation experiments and corroborating immunofluorescence studies. This polypeptide-internal routing information seems to be unique for the sequence of the trypanosome enzyme: a reporter protein fused to a Saccharomyces cerevisiae peptide containing the sequence corresponding to the 22-residue fragment of the T. brucei enzyme, was not targeted to glycosomes. In yeasts, as in most other organisms, TPI is indeed exclusively present in the cytosol. These results suggest that it may be possible to develop new trypanocidal drugs by targeting specifically the glycosome import mechanism of TPI. (C) 2010 Elsevier B.V. All rights reserved.

Journal Article Type Article
Publication Date 2010-05
Journal Molecular and Biochemical Parasitology
Print ISSN 0166-6851
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 171
Issue 1
Pages 45-49
APA6 Citation Galland, N., de Walque, S., Voncken, F. G., Verlinde, C. L., & Michels, P. A. (2010). An internal sequence targets Trypanosoma brucei triosephosphate isomerase to glycosomes. Molecular and Biochemical Parasitology, 171(1), (45-49). doi:10.1016/j.molbiopara.2010.01.002. ISSN 0166-6851
DOI https://doi.org/10.1016/j.molbiopara.2010.01.002
Keywords Molecular Biology; Parasitology
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