An internal sequence targets Trypanosoma brucei triosephosphate isomerase to glycosomes

Galland, Nathalie; de Walque, Stéphane; Voncken, Frank G.J.; Verlinde, Christophe L.M.J.; Michels, Paul A.M.

doi:10.1016/j.molbiopara.2010.01.002

An internal sequence targets Trypanosoma brucei triosephosphate isomerase to glycosomes

Galland, Nathalie; de Walque, Stéphane; Voncken, Frank G.J.; Verlinde, Christophe L.M.J.; Michels, Paul A.M.

Authors

Nathalie Galland

Stéphane de Walque

Dr Frank Voncken Frank.Voncken@hyms.ac.uk
Lecturer in Medical Microbiology, and Biomedical and Forensic Sciences. Biosafety Practitioner and Advisor.

Christophe L.M.J. Verlinde

Paul A.M. Michels

Abstract

In kinetoplastid protists, glycolysis is compartmentalized in glycosomes, organelles belonging to the peroxisome family. The Trypanosoma brucei glycosomal enzyme triosephosphate isomerase (TPI) does not contain either of the two established peroxisome-targeting signals, but we identified a 22 amino acids long fragment, present at an internal position of the polypeptide, that has the capacity to route a reporter protein to glycosomes in transfected trypanosomes, as demonstrated by cell-fractionation experiments and corroborating immunofluorescence studies. This polypeptide-internal routing information seems to be unique for the sequence of the trypanosome enzyme: a reporter protein fused to a Saccharomyces cerevisiae peptide containing the sequence corresponding to the 22-residue fragment of the T. brucei enzyme, was not targeted to glycosomes. In yeasts, as in most other organisms, TPI is indeed exclusively present in the cytosol. These results suggest that it may be possible to develop new trypanocidal drugs by targeting specifically the glycosome import mechanism of TPI. (C) 2010 Elsevier B.V. All rights reserved.

Citation

Galland, N., de Walque, S., Voncken, F. G., Verlinde, C. L., & Michels, P. A. (2010). An internal sequence targets Trypanosoma brucei triosephosphate isomerase to glycosomes. Molecular and Biochemical Parasitology, 171(1), 45-49. https://doi.org/10.1016/j.molbiopara.2010.01.002

Journal Article Type	Article
Acceptance Date	Jan 18, 2010
Online Publication Date	Feb 4, 2010
Publication Date	2010-05
Journal	MOLECULAR AND BIOCHEMICAL PARASITOLOGY
Print ISSN	0166-6851
Publisher	Elsevier
Peer Reviewed	Peer Reviewed
Volume	171
Issue	1
Pages	45-49
DOI	https://doi.org/10.1016/j.molbiopara.2010.01.002
Keywords	Molecular Biology; Parasitology
Public URL	https://hull-repository.worktribe.com/output/391577