P. Pena Diaz
Mitochondrial carrier family inventory of Trypanosoma brucei brucei: identification, expression and subcellular localisation
Diaz, P. Pena; Colasante, Claudia; Peña Diaz, P.; Clayton, Christine; Voncken, Frank
P. Peña Diaz
Dr Frank Voncken F.Voncken@hull.ac.uk
Lecturer in Medical Microbiology, and Biomedical and Forensic Sciences. Biosafety Practitioner and Advisor.
The mitochondrial carrier family (MCF) is a group of structurally conserved proteins that mediate the transport of a wide range of metabolic intermediates across the mitochondrial inner membrane. In this paper, an overview of the mitochondrial carrier proteins (MCPs) of the early-branching kinetoplastid parasite Trypanosoma brucei brucei is presented. Sequence analysis and phylogenetic reconstruction gave insight into the evolution and conservation of the 24 identified TbMCPs; for most of these, putative transport functions could be predicted. Comparison of the kinetoplastid MCP inventory to those previously reported for other eukaryotes revealed remarkable deviations: T. b. brucei lacks genes encoding some prototypical MCF members, such as the citrate carrier and uncoupling proteins. The in vivo expression of the identified TbMCPs in the two replicating life-cycle forms of T. b. brucei, the bloodstream-form and procyclic-form, was quantitatively assessed at the mRNA level by Northern blot analysis. Immunolocalisation studies confirmed that majority of the 24 identified TbMCPs is found in the mitochondrion of procyclic-form T. b. brucei. © 2009 Elsevier B.V. All rights reserved.
Colasante, C., Peña Diaz, P., Clayton, C., & Voncken, F. (2009). Mitochondrial carrier family inventory of Trypanosoma brucei brucei: identification, expression and subcellular localisation. Molecular and Biochemical Parasitology, 167(2), 104-117. https://doi.org/10.1016/j.molbiopara.2009.05.004
|Journal Article Type||Article|
|Acceptance Date||May 7, 2009|
|Online Publication Date||May 20, 2009|
|Journal||Molecular and biochemical parasitology|
|Peer Reviewed||Peer Reviewed|
|Keywords||Molecular Biology; Parasitology|
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