Mitochondrial carrier family inventory of Trypanosoma brucei brucei: identification, expression and subcellular localisation

Diaz, P. Pena; Colasante, Claudia; Peña Diaz, P.; Clayton, Christine; Voncken, Frank

Abstract

The mitochondrial carrier family (MCF) is a group of structurally conserved proteins that mediate the transport of a wide range of metabolic intermediates across the mitochondrial inner membrane. In this paper, an overview of the mitochondrial carrier proteins (MCPs) of the early-branching kinetoplastid parasite Trypanosoma brucei brucei is presented. Sequence analysis and phylogenetic reconstruction gave insight into the evolution and conservation of the 24 identified TbMCPs; for most of these, putative transport functions could be predicted. Comparison of the kinetoplastid MCP inventory to those previously reported for other eukaryotes revealed remarkable deviations: T. b. brucei lacks genes encoding some prototypical MCF members, such as the citrate carrier and uncoupling proteins. The in vivo expression of the identified TbMCPs in the two replicating life-cycle forms of T. b. brucei, the bloodstream-form and procyclic-form, was quantitatively assessed at the mRNA level by Northern blot analysis. Immunolocalisation studies confirmed that majority of the 24 identified TbMCPs is found in the mitochondrion of procyclic-form T. b. brucei. © 2009 Elsevier B.V. All rights reserved.

Citation

Colasante, C., Peña Diaz, P., Clayton, C., & Voncken, F. (2009). Mitochondrial carrier family inventory of Trypanosoma brucei brucei: identification, expression and subcellular localisation. Molecular and Biochemical Parasitology, 167(2), 104-117. https://doi.org/10.1016/j.molbiopara.2009.05.004

• Journal Article Type: Article
• Acceptance Date: May 7, 2009
• Online Publication Date: May 20, 2009
• Publication Date: 2009-10
• Journal: Molecular and biochemical parasitology
• Print ISSN: 0166-6851
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 167
• Issue: 2
• Pages: 104-117
• DOI: https://doi.org/10.1016/j.molbiopara.2009.05.004
• Keywords: Molecular Biology; Parasitology
• Public URL: https://hull-repository.worktribe.com/output/391599
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0166685109001406?via%3Dihub