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A microflow chemiluminescence system for determination of chloramphenicol in honey with preconcentration using a molecularly imprinted polymer

Liawruangath, Boonsom; Thongchai, Wisanu; Liawruangrath, Saisunee; Greenway, Gillian M.

Authors

Boonsom Liawruangath

Wisanu Thongchai

Saisunee Liawruangrath

Gillian M. Greenway



Abstract

A novel chemiluminescence (CL) microfluidic system incorporating a molecularly imprinted polymer (MIP) preconcentration step was used for the determination of chloramphenicol in honey samples. The MIP was prepared by using chloramphenicol as the template, diethylaminoethyl methacrylate (DAM) as the function monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linking monomer, 2, 2'-dimethoxy-2-phenylacetophenone (DMPA) as the free radical initiator and toluene and dodecanol as the solvent. The MIP was pre-loaded into a 10 mm long, 2 mm wide and 1501,,m deep channel in a planar glass microfluidic device. When the sample containing chloramphenicol was introduced into the microfluidic device it was first preconcentrated on the MIP then detected by an enhancement effect on the chemiluminescence reaction of tris(2, 2'-bipyridyl) ruthenium(II) with cerium(IV) sulphate in sulphuric acid. A micro-syringe pump was used to pump the reagents. The CL intensity was linear in relationship to the chloramphenicol concentrations from 1.55 x 10(-4) to 3.09 x 10(-3) m mol L-1 (r(2) = 0.9915) and the detection limit (3 sigma) and the quantitation limit (10 sigma)were found to be 7.46 x 10(-6) and 2.48 x 10(-5) mu mol L-1, respectively. This method offered a high selectivity and sensitivity for quantitative analysis of chloramphenicol in the honey samples. (C) 2010 Elsevier B.V. All rights reserved.

Citation

Thongchai, W., Liawruangath, B., Liawruangrath, S., & Greenway, G. M. (2010). A microflow chemiluminescence system for determination of chloramphenicol in honey with preconcentration using a molecularly imprinted polymer. Talanta, 82(2), 560-566. https://doi.org/10.1016/j.talanta.2010.05.007

Journal Article Type Article
Acceptance Date May 10, 2010
Online Publication Date May 16, 2010
Publication Date Jul 15, 2010
Journal TALANTA
Print ISSN 0039-9140
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 82
Issue 2
Pages 560-566
DOI https://doi.org/10.1016/j.talanta.2010.05.007
Keywords Chloramphenicol; Molecularly imprinted polymer (MIP); Chemiluminscence; Microfluidics; MicroFIA
Public URL https://hull-repository.worktribe.com/output/396310