Simultaneous analysis of micro-RNA and DNA for determining the body fluid origin of DNA profiles

van der Meer, Donny; Uchimoto, Mari L.; Williams, Graham

Abstract

Micro-RNAs (miRNAs) can be specifically expressed in forensically relevant body fluids such as blood or saliva. The aim of the study was to develop a simultaneous extraction and analysis protocol that allows for the acquisition of a DNA profile and the identity of the body fluid using a single process. DNA and micro-RNA were extracted from blood and saliva before undergoing a cDNA synthesis step by using stem-loop reverse transcription PCR. The resulting extracts containing DNA and cDNA synthesized from body fluid-specific miRNA markers then underwent standard STR analysis using a modified ABI AmpFℓSTR® NGM SElect™ kit. In all samples, a full DNA profile was obtained along with additional peaks corresponding to the miRNA marker targeted. In all cases, blood samples profiled exhibited a peak indicating the presence of the blood-specific miRNA marker and the saliva sample profiled exhibited a peak indicating the presence of the saliva-specific miRNA marker. © 2013 American Academy of Forensic Sciences.

Citation

van der Meer, D., Uchimoto, M. L., & Williams, G. (2013). Simultaneous analysis of micro-RNA and DNA for determining the body fluid origin of DNA profiles. Journal of Forensic Sciences, 58(4), 967-971. https://doi.org/10.1111/1556-4029.12160

• Journal Article Type: Article
• Acceptance Date: Jun 24, 2012
• Online Publication Date: May 17, 2013
• Publication Date: 2013-07
• Deposit Date: May 10, 2022
• Journal: Journal of Forensic Sciences
• Print ISSN: 0022-1198
• Electronic ISSN: 1556-4029
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 58
• Issue: 4
• Pages: 967-971
• DOI: https://doi.org/10.1111/1556-4029.12160
• Keywords: Forensic science; Micro-RNA; DNA; Body fluid identification; Co-isolation; Forensic genetics
• Public URL: https://hull-repository.worktribe.com/output/3993104