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Quantitative PCR analysis of blood- and saliva-specific microRNA markers following solid-phase DNA extraction

Omelia, Emma J.; Uchimoto, Mari L.; Williams, Graham


Emma J. Omelia

Mari L. Uchimoto


The use of mRNA for the identification of body fluids is of particular interest in forensic science, and increasing support has been demonstrated for the use of microRNA (miRNA) analysis. MiRNA is more stable than mRNA and has been shown to be differentially expressed in body fluids. No studies involving miRNA analysis from previously extracted DNA samples have yet been reported. The aim of this experiment was to determine if it was possible to conduct miRNA analysis on samples that were previously extracted using standard DNA extraction. Blood and saliva samples were extracted using DNA and RNA kits, followed by cDNA synthesis, and then underwent quantitative PCR analysis. A direct comparison of ΔCt values shows a larger abundance of miRNA following DNA extraction as opposed to total RNA extraction for both blood- and saliva-specific markers. By carrying out a comparison between the amounts of said markers, it could be seen that the expression of the blood-specific marker was higher in blood than in saliva, and vice versa for the saliva-specific marker. The results obtained could have a profound impact on cases for which the sample has already undergone DNA extraction, such as in cold cases. © 2013 Elsevier Inc. All rights reserved.


Omelia, E. J., Uchimoto, M. L., & Williams, G. (2013). Quantitative PCR analysis of blood- and saliva-specific microRNA markers following solid-phase DNA extraction. Analytical Biochemistry, 435(2), 120-122.

Journal Article Type Article
Acceptance Date Dec 22, 2012
Online Publication Date Jan 16, 2013
Publication Date Apr 15, 2013
Deposit Date May 10, 2022
Journal Analytical Biochemistry
Print ISSN 0003-2697
Electronic ISSN 1096-0309
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 435
Issue 2
Pages 120-122
Keywords Forensic genetics; Body fluid identification; MicroRNA; DNA extraction; Blood; Saliva
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