Sample introduction interface for on-chip nucleic acid-based analysis of Helicobacter pylori from stool samples

Esfahani, M. M. N.; Shaw, K. J.; Esfahani, M. M.N.; Kemp, C.; Tarn, M. D.; Melling, L.; Kemp, Cordula; Pamme, N.; Melling, Louise; Mosley, O.

Abstract

Despite recent advances in microfluidic-based integrated diagnostic systems, the sample introduction interface, especially with regards to large volume samples, has often been neglected. We present a sample introduction interface that allows direct on-chip processing of crude stool samples for the detection of Helicobacter pylori (H. pylori). The principle of IFAST (immiscible filtration assisted by surface tension) was adapted to include a large volume sample chamber with a septum-based interface for stool sample introduction. Solid chaotropic salt and dry superparamagnetic particles (PMPs) could be stored on-chip and reconstituted upon sample addition, simplifying the process of release of DNA from H. pylori cells and its binding to the PMPs. Finally, the PMPs were pulled via a magnet through a washing chamber containing an immiscible oil solution and into an elution chamber where the DNA was released into aqueous media for subsequent analysis. The entire process required only 7 min while enabling a 40-fold reduction in working volume from crude biological samples. The combination of a real-world interface and rapid DNA extraction offers the potential for the methodology to be used in point-of-care (POC) devices.

Citation

Esfahani, M. M. N., Shaw, K. J., Esfahani, M. M., Kemp, C., Tarn, M. D., Melling, L., Kemp, C., Pamme, N., Melling, L., & Mosley, O. (2016). Sample introduction interface for on-chip nucleic acid-based analysis of Helicobacter pylori from stool samples. Lab on a chip, 16(11), 2108-2115. https://doi.org/10.1039/c6lc00228e

• Journal Article Type: Article
• Conference Name: 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
• Acceptance Date: Apr 28, 2016
• Online Publication Date: May 10, 2016
• Publication Date: May 10, 2016
• Deposit Date: May 11, 2016
• Publicly Available Date: May 11, 2016
• Journal: Lab on a chip
• Print ISSN: 1473-0197
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 16
• Issue: 11
• Pages: 2108-2115
• ISBN: 9780979806490
• DOI: https://doi.org/10.1039/c6lc00228e
• Keywords: Helicobacter pylori, On-chip analysis , Nucleic acid-based analysis
• Public URL: https://hull-repository.worktribe.com/output/437825
• Publisher URL: http://pubs.rsc.org/en/content/articlelanding/2016/lc/c6lc00228e#!divAbstract
• Additional Information: This document is Similarity Check deposited; : Supplementary Information; : K. J. Shaw (ORCID); : The Royal Society of Chemistry has an exclusive publication licence for this journal; OPEN ACCESS: This article is freely available. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (CC BY 3.0); : Single-blind; : Received 18 February 2016; Accepted 28 April 2016; Advance Article published 10 May 2016; Version of Record published 24 May 2016
• Contract Date: May 11, 2016