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Sample introduction interface for on-chip nucleic acid-based analysis of Helicobacter pylori from stool samples

Esfahani, M. M. N.; Shaw, K. J.; Esfahani, M. M.N.; Kemp, C.; Tarn, M. D.; Melling, L.; Kemp, Cordula; Pamme, N.; Melling, Louise; Mosley, O.

Authors

M. M. N. Esfahani

K. J. Shaw

M. M.N. Esfahani

C. Kemp

M. D. Tarn

L. Melling

Cordula Kemp

N. Pamme

Louise Melling

O. Mosley



Abstract

Despite recent advances in microfluidic-based integrated diagnostic systems, the sample introduction interface, especially with regards to large volume samples, has often been neglected. We present a sample introduction interface that allows direct on-chip processing of crude stool samples for the detection of Helicobacter pylori (H. pylori). The principle of IFAST (immiscible filtration assisted by surface tension) was adapted to include a large volume sample chamber with a septum-based interface for stool sample introduction. Solid chaotropic salt and dry superparamagnetic particles (PMPs) could be stored on-chip and reconstituted upon sample addition, simplifying the process of release of DNA from H. pylori cells and its binding to the PMPs. Finally, the PMPs were pulled via a magnet through a washing chamber containing an immiscible oil solution and into an elution chamber where the DNA was released into aqueous media for subsequent analysis. The entire process required only 7 min while enabling a 40-fold reduction in working volume from crude biological samples. The combination of a real-world interface and rapid DNA extraction offers the potential for the methodology to be used in point-of-care (POC) devices.

Citation

Esfahani, M. M. N., Shaw, K. J., Esfahani, M. M., Kemp, C., Tarn, M. D., Melling, L., …Mosley, O. (2016). Sample introduction interface for on-chip nucleic acid-based analysis of Helicobacter pylori from stool samples. Lab on a chip, 16(11), 2108-2115. https://doi.org/10.1039/c6lc00228e

Journal Article Type Article
Conference Name 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Acceptance Date Apr 28, 2016
Online Publication Date May 10, 2016
Publication Date May 10, 2016
Deposit Date May 11, 2016
Publicly Available Date May 11, 2016
Journal Lab on a chip
Print ISSN 1473-0197
Electronic ISSN 1473-0189
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 16
Issue 11
Pages 2108-2115
ISBN 9780979806490
DOI https://doi.org/10.1039/c6lc00228e
Keywords Helicobacter pylori, On-chip analysis , Nucleic acid-based analysis
Public URL https://hull-repository.worktribe.com/output/437825
Publisher URL http://pubs.rsc.org/en/content/articlelanding/2016/lc/c6lc00228e#!divAbstract
Additional Information This document is Similarity Check deposited; : Supplementary Information; : K. J. Shaw (ORCID); : The Royal Society of Chemistry has an exclusive publication licence for this journal; OPEN ACCESS: This article is freely available. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (CC BY 3.0); : Single-blind; : Received 18 February 2016; Accepted 28 April 2016; Advance Article published 10 May 2016; Version of Record published 24 May 2016

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