The use of carrier RNA to enhance DNA extraction from microfluidic-based silica monoliths

Dyer, Charlotte E.; Shaw, Kirsty J.; Thain, Lauren; Dyer, Charlotte; Docker, Peter T.; Greenman, John; Greenway, Gillian M.; Haswell, Stephen J.

Abstract

DNA extraction was carried out on silica-based monoliths within a microfluidic device. Solid-phase DNA extraction methodology was applied in which the DNA binds to silica in the presence of a chaotropic salt, such as guanidine hydrochloride, and is eluted in a low ionic strength solution, such as water. The addition of poly-A carrier RNA to the chaotropic salt solution resulted in a marked increase in the effective amount of DNA that could be recovered (25 ng) compared to the absence of RNA (5 ng) using the silica-based monolith. These findings confirm that techniques utilising nucleic acid carrier molecules can enhance DNA extraction methodologies in microfluidic applications. (C) 2009 Elsevier B.V. All rights reserved.

Citation

Shaw, K. J., Thain, L., Dyer, C., Docker, P. T., Greenman, J., Greenway, G. M., & Haswell, S. J. (2009). The use of carrier RNA to enhance DNA extraction from microfluidic-based silica monoliths. Analytica Chimica Acta, 652(1-2), 231-233. https://doi.org/10.1016/j.aca.2009.03.038

• Journal Article Type: Article
• Acceptance Date: Mar 24, 2009
• Online Publication Date: Mar 31, 2009
• Publication Date: Oct 12, 2009
• Journal: ANALYTICA CHIMICA ACTA
• Print ISSN: 0003-2670
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 652
• Issue: 1-2
• Pages: 231-233
• DOI: https://doi.org/10.1016/j.aca.2009.03.038
• Keywords: Analytical Chemistry; Spectroscopy; Biochemistry; Environmental Chemistry
• Public URL: https://hull-repository.worktribe.com/output/391623
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0003267009004450?via%3Dihub