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The use of carrier RNA to enhance DNA extraction from microfluidic-based silica monoliths

Dyer, Charlotte E.; Shaw, Kirsty J.; Thain, Lauren; Dyer, Charlotte; Docker, Peter T.; Greenman, John; Greenway, Gillian M.; Haswell, Stephen J.

Authors

Charlotte E. Dyer

Kirsty J. Shaw

Lauren Thain

Charlotte Dyer C.E.Dyer@hull.ac.uk

Peter T. Docker

Gillian M. Greenway G.M.Greenway@hull.ac.uk

Stephen J. Haswell



Abstract

DNA extraction was carried out on silica-based monoliths within a microfluidic device. Solid-phase DNA extraction methodology was applied in which the DNA binds to silica in the presence of a chaotropic salt, such as guanidine hydrochloride, and is eluted in a low ionic strength solution, such as water. The addition of poly-A carrier RNA to the chaotropic salt solution resulted in a marked increase in the effective amount of DNA that could be recovered (25 ng) compared to the absence of RNA (5 ng) using the silica-based monolith. These findings confirm that techniques utilising nucleic acid carrier molecules can enhance DNA extraction methodologies in microfluidic applications. (C) 2009 Elsevier B.V. All rights reserved.

Citation

Shaw, K. J., Thain, L., Dyer, C., Docker, P. T., Greenman, J., Greenway, G. M., & Haswell, S. J. (2009). The use of carrier RNA to enhance DNA extraction from microfluidic-based silica monoliths. Analytica Chimica Acta, 652(1-2), 231-233. https://doi.org/10.1016/j.aca.2009.03.038

Journal Article Type Article
Acceptance Date Mar 24, 2009
Online Publication Date Mar 31, 2009
Publication Date Oct 12, 2009
Journal ANALYTICA CHIMICA ACTA
Print ISSN 0003-2670
Electronic ISSN 1873-4324
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 652
Issue 1-2
Pages 231-233
DOI https://doi.org/10.1016/j.aca.2009.03.038
Keywords Analytical Chemistry; Spectroscopy; Biochemistry; Environmental Chemistry
Public URL https://hull-repository.worktribe.com/output/391623
Publisher URL https://www.sciencedirect.com/science/article/pii/S0003267009004450?via%3Dihub