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Rapid PCR amplification using a microfluidic device with integrated microwave heating and air impingement cooling

Dyer, Charlotte E.; Shaw, Kirsty J.; Docker, Peter T.; Yelland, John V.; Dyer, Charlotte E.; Greenman, John; Greenway, Gillian M.; Haswell, Stephen J.

Authors

Charlotte E. Dyer

Kirsty J. Shaw

Peter T. Docker

John V. Yelland

Charlotte E. Dyer C.E.Dyer@hull.ac.uk

Gillian M. Greenway G.M.Greenway@hull.ac.uk

Stephen J. Haswell



Abstract

A microwave heating system is described for performing polymerase chain reaction (PCR) in a microfluidic device. The heating system, in combination with air impingement cooling, provided rapid thermal cycling with heating and cooling rates of up to 65 degrees C s(-1) and minimal over- or under-shoot (+/- 0.1 degrees C) when reaching target temperatures. In addition, once the required temperature was reached it could be maintained with an accuracy of +/- 0.1 degrees C. To demonstrate the functionality of the system, PCR was successfully performed for the amplification of the Amelogenin locus using heating rates and quantities an order of magnitude faster and smaller than current commercial instruments.

Journal Article Type Article
Publication Date Apr 23, 2010
Journal LAB ON A CHIP
Print ISSN 1473-0197
Electronic ISSN 1473-0189
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 10
Issue 13
Pages 1725-1728
APA6 Citation Shaw, K. J., Docker, P. T., Yelland, J. V., Dyer, C. E., Greenman, J., Greenway, G. M., & Haswell, S. J. (2010). Rapid PCR amplification using a microfluidic device with integrated microwave heating and air impingement cooling. Lab on a chip, 10(13), 1725-1728. https://doi.org/10.1039/c000357n
DOI https://doi.org/10.1039/c000357n
Keywords Nucleic-acid analysis system chips
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