Rapid PCR amplification using a microfluidic device with integrated microwave heating and air impingement cooling

Dyer, Charlotte E.; Shaw, Kirsty J.; Docker, Peter T.; Yelland, John V.; Dyer, Charlotte E.; Greenman, John; Greenway, Gillian M.; Haswell, Stephen J.

Abstract

A microwave heating system is described for performing polymerase chain reaction (PCR) in a microfluidic device. The heating system, in combination with air impingement cooling, provided rapid thermal cycling with heating and cooling rates of up to 65 degrees C s(-1) and minimal over- or under-shoot (+/- 0.1 degrees C) when reaching target temperatures. In addition, once the required temperature was reached it could be maintained with an accuracy of +/- 0.1 degrees C. To demonstrate the functionality of the system, PCR was successfully performed for the amplification of the Amelogenin locus using heating rates and quantities an order of magnitude faster and smaller than current commercial instruments.

Citation

Shaw, K. J., Docker, P. T., Yelland, J. V., Dyer, C. E., Greenman, J., Greenway, G. M., & Haswell, S. J. (2010). Rapid PCR amplification using a microfluidic device with integrated microwave heating and air impingement cooling. Lab on a chip, 10(13), 1725-1728. https://doi.org/10.1039/c000357n

• Journal Article Type: Article
• Acceptance Date: Apr 6, 2010
• Online Publication Date: Apr 23, 2010
• Publication Date: Apr 23, 2010
• Journal: LAB ON A CHIP
• Print ISSN: 1473-0197
• Electronic ISSN: 1473-0189
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 10
• Issue: 13
• Pages: 1725-1728
• DOI: https://doi.org/10.1039/c000357n
• Keywords: Nucleic-acid analysis system chips
• Public URL: https://hull-repository.worktribe.com/output/396312