Kirsty J. Shaw
Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device
Shaw, Kirsty J.; Docker, Peter T.; Haswell, Stephen J.; Joyce, Domino A.; Dyer, Charlotte E.; Greenman, John; Greenway, Gillian M.
Authors
Peter T. Docker
Stephen J. Haswell
Dr Domino Joyce D.Joyce@hull.ac.uk
Senior Lecturer
Charlotte E. Dyer C.E.Dyer@hull.ac.uk
Professor John Greenman J.Greenman@hull.ac.uk
Professor of Tumour Immunology
Gillian M. Greenway G.M.Greenway@hull.ac.uk
Abstract
A novel DNA loading methodology is presented for performing DNA extraction on a microfluidic system. DNA in a chaotropic salt solution was manually loaded onto a silica monolith orthogonal to the subsequent flow of wash and elution solutions. DNA was successfully extracted from buccal swabs using electro-osmotic pumping (EOP) coupled with in situ reagents contained within a 1.5% agarose gel matrix. The extracted DNA was of sufficient quantity and purity for polymerase chain reaction (PCR) amplification.
Citation
Shaw, K. J., Docker, P. T., Haswell, S. J., Joyce, D. A., Dyer, C. E., Greenman, J., & Greenway, G. M. (2009). Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device. Lab on a chip, 9(23), 3430-3432. https://doi.org/10.1039/b913309g
Journal Article Type | Article |
---|---|
Publication Date | Jan 1, 2009 |
Journal | LAB ON A CHIP |
Print ISSN | 1473-0197 |
Electronic ISSN | 1473-0189 |
Publisher | Royal Society of Chemistry |
Peer Reviewed | Peer Reviewed |
Volume | 9 |
Issue | 23 |
Pages | 3430-3432 |
DOI | https://doi.org/10.1039/b913309g |
Keywords | Biochemistry; Bioengineering; General Chemistry; Biomedical Engineering |
Public URL | https://hull-repository.worktribe.com/output/391625 |
This file is under embargo due to copyright reasons.
You might also like
Interactive lectures: Clickers or personal devices?
(2015)
Journal Article
Secondary contact seeds phenotypic novelty in cichlid fishes
(2015)
Journal Article
Gene duplication in an African cichlid adaptive radiation
(2014)
Journal Article