Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device

Shaw, Kirsty J.; Docker, Peter T.; Haswell, Stephen J.; Joyce, Domino A.; Dyer, Charlotte E.; Greenman, John; Greenway, Gillian M.

doi:10.1039/b913309g

Authors

Kirsty J. Shaw

Peter T. Docker

Stephen J. Haswell

Dr Domino Joyce D.Joyce@hull.ac.uk
Senior Lecturer

Charlotte E. Dyer C.E.Dyer@hull.ac.uk

Professor John Greenman J.Greenman@hull.ac.uk
Professor of Tumour Immunology

Gillian M. Greenway G.M.Greenway@hull.ac.uk

Abstract

A novel DNA loading methodology is presented for performing DNA extraction on a microfluidic system. DNA in a chaotropic salt solution was manually loaded onto a silica monolith orthogonal to the subsequent flow of wash and elution solutions. DNA was successfully extracted from buccal swabs using electro-osmotic pumping (EOP) coupled with in situ reagents contained within a 1.5% agarose gel matrix. The extracted DNA was of sufficient quantity and purity for polymerase chain reaction (PCR) amplification.

Citation

Shaw, K. J., Docker, P. T., Haswell, S. J., Joyce, D. A., Dyer, C. E., Greenman, J., & Greenway, G. M. (2009). Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device. Lab on a chip, 9(23), 3430-3432. https://doi.org/10.1039/b913309g

Journal Article Type	Article
Publication Date	Jan 1, 2009
Publicly Available Date
Journal	LAB ON A CHIP
Print ISSN	1473-0197
Electronic ISSN	1473-0189
Publisher	Royal Society of Chemistry
Peer Reviewed	Peer Reviewed
Volume	9
Issue	23
Pages	3430-3432
DOI	https://doi.org/10.1039/b913309g
Keywords	Biochemistry; Bioengineering; General Chemistry; Biomedical Engineering
Public URL	https://hull-repository.worktribe.com/output/391625