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Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device

Shaw, Kirsty J.; Docker, Peter T.; Haswell, Stephen J.; Joyce, Domino A.; Dyer, Charlotte E.; Greenman, John; Greenway, Gillian M.

Authors

Kirsty J. Shaw

Peter T. Docker

Stephen J. Haswell

Charlotte E. Dyer C.E.Dyer@hull.ac.uk

Gillian M. Greenway G.M.Greenway@hull.ac.uk



Abstract

A novel DNA loading methodology is presented for performing DNA extraction on a microfluidic system. DNA in a chaotropic salt solution was manually loaded onto a silica monolith orthogonal to the subsequent flow of wash and elution solutions. DNA was successfully extracted from buccal swabs using electro-osmotic pumping (EOP) coupled with in situ reagents contained within a 1.5% agarose gel matrix. The extracted DNA was of sufficient quantity and purity for polymerase chain reaction (PCR) amplification.

Journal Article Type Article
Publication Date Jan 1, 2009
Journal LAB ON A CHIP
Print ISSN 1473-0197
Electronic ISSN 1473-0189
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 9
Issue 23
Pages 3430-3432
APA6 Citation Shaw, K. J., Docker, P. T., Haswell, S. J., Joyce, D. A., Dyer, C. E., Greenman, J., & Greenway, G. M. (2009). Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device. Lab on a chip, 9(23), 3430-3432. https://doi.org/10.1039/b913309g
DOI https://doi.org/10.1039/b913309g
Keywords Biochemistry; Bioengineering; General Chemistry; Biomedical Engineering

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