Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device

Shaw, Kirsty J.; Docker, Peter T.; Haswell, Stephen J.; Joyce, Domino A.; Dyer, Charlotte E.; Greenman, John; Greenway, Gillian M.

Abstract

A novel DNA loading methodology is presented for performing DNA extraction on a microfluidic system. DNA in a chaotropic salt solution was manually loaded onto a silica monolith orthogonal to the subsequent flow of wash and elution solutions. DNA was successfully extracted from buccal swabs using electro-osmotic pumping (EOP) coupled with in situ reagents contained within a 1.5% agarose gel matrix. The extracted DNA was of sufficient quantity and purity for polymerase chain reaction (PCR) amplification.

Citation

Shaw, K. J., Docker, P. T., Haswell, S. J., Joyce, D. A., Dyer, C. E., Greenman, J., & Greenway, G. M. (2009). Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device. Lab on a chip, 9(23), 3430-3432. https://doi.org/10.1039/b913309g

• Journal Article Type: Article
• Publication Date: Jan 1, 2009
• Journal: LAB ON A CHIP
• Print ISSN: 1473-0197
• Electronic ISSN: 1473-0189
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 9
• Issue: 23
• Pages: 3430-3432
• DOI: https://doi.org/10.1039/b913309g
• Keywords: Biochemistry; Bioengineering; General Chemistry; Biomedical Engineering
• Public URL: https://hull-repository.worktribe.com/output/391625