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Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device

Shaw, Kirsty J.; Docker, Peter T.; Haswell, Stephen J.; Joyce, Domino A.; Dyer, Charlotte E.; Greenman, John; Greenway, Gillian M.

Authors

Kirsty J. Shaw

Peter T. Docker

Stephen J. Haswell

Charlotte E. Dyer C.E.Dyer@hull.ac.uk

Gillian M. Greenway G.M.Greenway@hull.ac.uk



Abstract

A novel DNA loading methodology is presented for performing DNA extraction on a microfluidic system. DNA in a chaotropic salt solution was manually loaded onto a silica monolith orthogonal to the subsequent flow of wash and elution solutions. DNA was successfully extracted from buccal swabs using electro-osmotic pumping (EOP) coupled with in situ reagents contained within a 1.5% agarose gel matrix. The extracted DNA was of sufficient quantity and purity for polymerase chain reaction (PCR) amplification.

Citation

Shaw, K. J., Docker, P. T., Haswell, S. J., Joyce, D. A., Dyer, C. E., Greenman, J., & Greenway, G. M. (2009). Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device. Lab on a chip, 9(23), 3430-3432. https://doi.org/10.1039/b913309g

Journal Article Type Article
Publication Date Jan 1, 2009
Publicly Available Date
Journal LAB ON A CHIP
Print ISSN 1473-0197
Electronic ISSN 1473-0189
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 9
Issue 23
Pages 3430-3432
DOI https://doi.org/10.1039/b913309g
Keywords Biochemistry; Bioengineering; General Chemistry; Biomedical Engineering
Public URL https://hull-repository.worktribe.com/output/391625