Kirsty J. Shaw
Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device
Shaw, Kirsty J.; Docker, Peter T.; Haswell, Stephen J.; Joyce, Domino A.; Dyer, Charlotte E.; Greenman, John; Greenway, Gillian M.
Peter T. Docker
Stephen J. Haswell
Dr Domino Joyce D.Joyce@hull.ac.uk
Charlotte E. Dyer C.E.Dyer@hull.ac.uk
Professor John Greenman J.Greenman@hull.ac.uk
Professor of Tumour Immunology
Gillian M. Greenway G.M.Greenway@hull.ac.uk
A novel DNA loading methodology is presented for performing DNA extraction on a microfluidic system. DNA in a chaotropic salt solution was manually loaded onto a silica monolith orthogonal to the subsequent flow of wash and elution solutions. DNA was successfully extracted from buccal swabs using electro-osmotic pumping (EOP) coupled with in situ reagents contained within a 1.5% agarose gel matrix. The extracted DNA was of sufficient quantity and purity for polymerase chain reaction (PCR) amplification.
Shaw, K. J., Docker, P. T., Haswell, S. J., Joyce, D. A., Dyer, C. E., Greenman, J., & Greenway, G. M. (2009). Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device. Lab on a chip, 9(23), 3430-3432. https://doi.org/10.1039/b913309g
|Journal Article Type||Article|
|Publication Date||Jan 1, 2009|
|Journal||LAB ON A CHIP|
|Publisher||Royal Society of Chemistry|
|Peer Reviewed||Peer Reviewed|
|Keywords||Biochemistry; Bioengineering; General Chemistry; Biomedical Engineering|
This file is under embargo due to copyright reasons.
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