Cellular internalisation, bioimaging and dark and photodynamic cytotoxicity of silica nanoparticles doped by {Mo₆I₈}⁴⁺ metal clusters

Solovieva, Anastasiya O.; Vorotnikov, Yuri A.; Trifonova, Kristina E.; Efremova, Olga A.; Krasilnikova, Anna A.; Brylev, Konstantin A.; Vorontsova, Elena V.; Avrorov, Pavel A.; Shestopalova, Lidiya V.; Poveshchenko, Alexander F.; Mironov, Yuri V.; Shestopalov, Michael A.

Abstract

Silica nanoparticles (SNPs) doped by hexanuclear molybdenum cluster complexes [{Mo₆X₈}L₆]n (X = Cl, Br, or I; L = various inorganic or organic ligands) have been recently suggested as materials with a high potential for biomedical applications due to both the outstanding photoluminescent properties and the ability to efficiently generate singlet oxygen upon photoirradiation. However, no studies were undertaken so far to prove this concept. Therefore, here we examined the potential of photoluminescent SNPs doped by {Mo₆I₈}⁴⁺ for such applications as bioimaging and photodynamic therapy using human epidermoid larynx carcinoma (Hep-2) cell line as a model. Our results demonstrated both: (i) significant luminescence from cells with internalised molybdenum cluster doped SNPs combined with the low cytotoxicity of particles in the darkness and (ii) significant cytotoxicity of the particles upon photoirradiation. Thus, this research provides strong experimental evidence for high potential of molybdenum cluster doped materials in such biomedical applications as optical bioimaging, biolabeling and photodynamic therapy.

Citation

Solovieva, A. O., Vorotnikov, Y. A., Trifonova, K. E., Efremova, O. A., Krasilnikova, A. A., Brylev, K. A., Vorontsova, E. V., Avrorov, P. A., Shestopalova, L. V., Poveshchenko, A. F., Mironov, Y. V., & Shestopalov, M. A. (in press). Cellular internalisation, bioimaging and dark and photodynamic cytotoxicity of silica nanoparticles doped by {Mo₆I₈}⁴⁺ metal clusters. Journal of Materials Chemistry B, 4(28), 4839-4846. https://doi.org/10.1039/c6tb00723f

• Journal Article Type: Article
• Acceptance Date: Jun 13, 2016
• Online Publication Date: Jun 14, 2016
• Deposit Date: Jun 20, 2016
• Publicly Available Date: Jun 20, 2016
• Journal: Journal of materials chemistry B
• Print ISSN: 2050-750X
• Electronic ISSN: 2050-7518
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 4
• Issue: 28
• Pages: 4839-4846
• DOI: https://doi.org/10.1039/c6tb00723f
• Keywords: Silica nanoparticles
• Public URL: https://hull-repository.worktribe.com/output/439842
• Publisher URL: http://pubs.rsc.org/en/content/articlelanding/2016/tb/c6tb00723f#!divAbstract
• Additional Information: : This document is Similarity Check deposited; : Supplementary Information; : Michael A. Shestopalov (ORCID); : Michael A. Shestopalov (ResearcherID); : The Royal Society of Chemistry has an exclusive publication licence for this journal; OPEN ACCESS: This article is freely available. This article is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported Licence (CC BY-NC 3.0); : Single-blind; : Received 21 March 2016; Accepted 13 June 2016; Accepted Manuscript published 14 June 2016; Advance Article published 27 June 2016; Version of Record published 13 July 2016
• Contract Date: Jun 20, 2016