Impact of Surface Ligand on the Biocompatibility of InP/ZnS Quantum Dots with Platelets

Gil, Hélio M.; Booth, Zoe; Price, Thomas W.; Lee, Jessica; Naylor‐Adamson, Leigh; Avery, Michelle; Muravitskaya, Alina; Hondow, Nicole; Allsup, David; Schneider, Jürgen E.; Naseem, Khalid; Adawi, Ali M.; Bouillard, Jean Sebastien G.; Chamberlain, Thomas W.; Calaminus, Simon D.J.; Stasiuk, Graeme J.

Abstract

InP/ZnS quantum dots (QDs) have received a large focus in recent years as a safer alternative to heavy metal-based QDs. Given their intrinsic fluorescent imaging capabilities, these QDs can be potentially relevant for in vivo platelet imaging. The InP/ZnS QDs are synthesized and their biocompatibility investigated through the use of different phase transfer agents. Analysis of platelet function indicates that platelet-QD interaction can occur at all concentrations and for all QD permutations tested. However, as the QD concentration increases, platelet aggregation is induced by QDs alone independent of natural platelet agonists. This study helps to define a range of concentrations and coatings (thioglycolic acid and penicillamine) that are biocompatible with platelet function. With this information, the platelet-QD interaction can be identified using multiple methods. Fluorescent lifetime imaging microscopy (FLIM) and confocal studies have shown QDs localize on the surface of the platelet toward the center while showing evidence of energy transfer within the QD population. It is believed that these findings are an important stepping point for the development of fluorescent probes for platelet imaging.

Citation

Gil, H. M., Booth, Z., Price, T. W., Lee, J., Naylor‐Adamson, L., Avery, M., Muravitskaya, A., Hondow, N., Allsup, D., Schneider, J. E., Naseem, K., Adawi, A. M., Bouillard, J. S. G., Chamberlain, T. W., Calaminus, S. D., & Stasiuk, G. J. (in press). Impact of Surface Ligand on the Biocompatibility of InP/ZnS Quantum Dots with Platelets. Small, Article 2304881. https://doi.org/10.1002/smll.202304881

• Journal Article Type: Article
• Acceptance Date: Oct 26, 2023
• Online Publication Date: Nov 9, 2023
• Deposit Date: Nov 15, 2023
• Publicly Available Date: Nov 16, 2023
• Journal: Small
• Print ISSN: 1613-6810
• Electronic ISSN: 1613-6829
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Article Number: 2304881
• DOI: https://doi.org/10.1002/smll.202304881
• Keywords: Fluorescence lifetime imaging microscopy (FLIM); Fluorescence; Hot-injections; InP/ZnS quantum dots (QDs); Platelet imaging; Platelets biocompatibility
• Public URL: https://hull-repository.worktribe.com/output/4443974