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A microfluidic chip based model for the study of full thickness human intestinal tissue using dual flow

Dawson, A.; Dyer, C.; Macfie, J.; Davies, J.; Karsai, L.; Greenman, J.; Jacobsen, M.

Authors

A. Dawson

C. Dyer

J. Macfie

J. Davies

L. Karsai

M. Jacobsen



Abstract

© 2016 Author(s). The study of inflammatory bowel disease, including Ulcerative Colitis and Crohn's Disease, has relied largely upon the use of animal or cell culture models; neither of which can represent all aspects of the human pathophysiology. Presented herein is a dual flow microfluidic device which holds full thickness human intestinal tissue in a known orientation. The luminal and serosal sides are independently perfused ex vivo with nutrients with simultaneous waste removal for up to 72 h. The microfluidic device maintains the viability and integrity of the tissue as demonstrated through Haematoxylin & Eosin staining, immunohistochemistry and release of lactate dehydrogenase. In addition, the inflammatory state remains in the tissue after perfusion on the device as determined by measuring calprotectin levels. It is anticipated that this human model will be extremely useful for studying the biology and tes ting novel interventions in diseased tissue.

Journal Article Type Article
Publication Date Nov 1, 2016
Journal Biomicrofluidics
Electronic ISSN 1932-1058
Publisher AIP Publishing
Peer Reviewed Peer Reviewed
Volume 10
Issue 6
Article Number ARTN 064101
Pages 064101
APA6 Citation Dawson, A., Dyer, C., Macfie, J., Davies, J., Karsai, L., Greenman, J., & Jacobsen, M. (2016). A microfluidic chip based model for the study of full thickness human intestinal tissue using dual flow. Biomicrofluidics, 10(6), 064101. https://doi.org/10.1063/1.4964813
DOI https://doi.org/10.1063/1.4964813
Keywords Inflammatory bowel diseases; Cell culture; Pathological physiology; Eosin; Microfluidic devices
Publisher URL http://aip.scitation.org/doi/10.1063/1.4964813
Copyright Statement ©2017 University of Hull
Additional Information Authors' accepted manuscript of article of an article which has been published in: Biomicrofluidics, 2016, v.10, issue 6.

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