A microfluidic chip based model for the study of full thickness human intestinal tissue using dual flow
Dawson, A.; Dyer, C.; Macfie, J.; Davies, J.; Karsai, L.; Greenman, J.; Jacobsen, M.
Professor John Greenman J.Greenman@hull.ac.uk
Professor of Tumour Immunology
© 2016 Author(s). The study of inflammatory bowel disease, including Ulcerative Colitis and Crohn's Disease, has relied largely upon the use of animal or cell culture models; neither of which can represent all aspects of the human pathophysiology. Presented herein is a dual flow microfluidic device which holds full thickness human intestinal tissue in a known orientation. The luminal and serosal sides are independently perfused ex vivo with nutrients with simultaneous waste removal for up to 72 h. The microfluidic device maintains the viability and integrity of the tissue as demonstrated through Haematoxylin & Eosin staining, immunohistochemistry and release of lactate dehydrogenase. In addition, the inflammatory state remains in the tissue after perfusion on the device as determined by measuring calprotectin levels. It is anticipated that this human model will be extremely useful for studying the biology and tes ting novel interventions in diseased tissue.
|Journal Article Type||Article|
|Publication Date||Nov 1, 2016|
|Peer Reviewed||Peer Reviewed|
|Article Number||ARTN 064101|
|APA6 Citation||Dawson, A., Dyer, C., Macfie, J., Davies, J., Karsai, L., Greenman, J., & Jacobsen, M. (2016). A microfluidic chip based model for the study of full thickness human intestinal tissue using dual flow. Biomicrofluidics, 10(6), 064101. https://doi.org/10.1063/1.4964813|
|Keywords||Inflammatory bowel diseases; Cell culture; Pathological physiology; Eosin; Microfluidic devices|
|Copyright Statement||©2017 University of Hull|
|Additional Information||Authors' accepted manuscript of article of an article which has been published in: Biomicrofluidics, 2016, v.10, issue 6.|
©2017 University of Hull