A microfluidic chip based model for the study of full thickness human intestinal tissue using dual flow

Dawson, A.; Dyer, C.; Macfie, J.; Davies, J.; Karsai, L.; Greenman, J.; Jacobsen, M.

Abstract

© 2016 Author(s). The study of inflammatory bowel disease, including Ulcerative Colitis and Crohn's Disease, has relied largely upon the use of animal or cell culture models; neither of which can represent all aspects of the human pathophysiology. Presented herein is a dual flow microfluidic device which holds full thickness human intestinal tissue in a known orientation. The luminal and serosal sides are independently perfused ex vivo with nutrients with simultaneous waste removal for up to 72 h. The microfluidic device maintains the viability and integrity of the tissue as demonstrated through Haematoxylin & Eosin staining, immunohistochemistry and release of lactate dehydrogenase. In addition, the inflammatory state remains in the tissue after perfusion on the device as determined by measuring calprotectin levels. It is anticipated that this human model will be extremely useful for studying the biology and tes ting novel interventions in diseased tissue.

Citation

Dawson, A., Dyer, C., Macfie, J., Davies, J., Karsai, L., Greenman, J., & Jacobsen, M. (2016). A microfluidic chip based model for the study of full thickness human intestinal tissue using dual flow. Biomicrofluidics, 10(6), 064101. https://doi.org/10.1063/1.4964813

• Journal Article Type: Article
• Acceptance Date: Sep 30, 2016
• Online Publication Date: Nov 1, 2016
• Publication Date: Nov 1, 2016
• Deposit Date: Jan 11, 2017
• Publicly Available Date: Dec 13, 2017
• Journal: Biomicrofluidics
• Electronic ISSN: 1932-1058
• Publisher: American Institute of Physics
• Peer Reviewed: Peer Reviewed
• Volume: 10
• Issue: 6
• Article Number: ARTN 064101
• Pages: 064101
• DOI: https://doi.org/10.1063/1.4964813
• Keywords: Inflammatory bowel diseases; Cell culture; Pathological physiology; Eosin; Microfluidic devices
• Public URL: https://hull-repository.worktribe.com/output/447009
• Publisher URL: http://aip.scitation.org/doi/10.1063/1.4964813
• Additional Information: Authors' accepted manuscript of article of an article which has been published in: Biomicrofluidics, 2016, v.10, issue 6.
• Contract Date: Jan 11, 2017