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Direct processing of clinically relevant large volume samples for the detection of sexually transmitted infectious agents from urine on a microfluidic device

Kemp, Cordula; Birch, Christopher; Shaw, Kirsty J.; Nixon, Gavin J.; Docker, Peter T.; Greenman, John; Dyer, Charlotte; Huggett, Jim F.; Haswell, Stephen J.; Foy, Carole A.; Dyer, Charlotte E.

Authors

Cordula Kemp Cordula.Kemp@hull.ac.uk

Christopher Birch

Kirsty J. Shaw

Gavin J. Nixon

Peter T. Docker

Charlotte Dyer C.E.Dyer@hull.ac.uk

Jim F. Huggett

Stephen J. Haswell

Carole A. Foy

Charlotte E. Dyer



Abstract

Urine is a preferred specimen for nucleic acid-based detection of sexually transmitted infections (STIs) but represents a challenge for microfluidic devices due to low analyte concentrations. We present an extraction methodology enabling rapid on-chip nucleic acid purification directly from clinically relevant sample volumes up to 1 ml and subsequent PCR amplification detection.

Citation

Kemp, C., Birch, C., Shaw, K. J., Nixon, G. J., Docker, P. T., Greenman, J., …Dyer, C. E. (2012). Direct processing of clinically relevant large volume samples for the detection of sexually transmitted infectious agents from urine on a microfluidic device. Analytical Methods, 4(7), 2141-2144. doi:10.1039/c2ay25075f

Journal Article Type Article
Acceptance Date May 9, 2012
Online Publication Date May 10, 2012
Publication Date Jul 1, 2012
Journal ANALYTICAL METHODS
Print ISSN 1759-9660
Electronic ISSN 1759-9679
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 4
Issue 7
Pages 2141-2144
DOI https://doi.org/10.1039/c2ay25075f
Keywords General Engineering; Analytical Chemistry; General Chemical Engineering
Public URL https://hull-repository.worktribe.com/output/417821