Direct processing of clinically relevant large volume samples for the detection of sexually transmitted infectious agents from urine on a microfluidic device

Kemp, Cordula; Birch, Christopher; Shaw, Kirsty J.; Nixon, Gavin J.; Docker, Peter T.; Greenman, John; Dyer, Charlotte; Huggett, Jim F.; Haswell, Stephen J.; Foy, Carole A.; Dyer, Charlotte E.

Abstract

Urine is a preferred specimen for nucleic acid-based detection of sexually transmitted infections (STIs) but represents a challenge for microfluidic devices due to low analyte concentrations. We present an extraction methodology enabling rapid on-chip nucleic acid purification directly from clinically relevant sample volumes up to 1 ml and subsequent PCR amplification detection.

Citation

Kemp, C., Birch, C., Shaw, K. J., Nixon, G. J., Docker, P. T., Greenman, J., Huggett, J. F., Haswell, S. J., Foy, C. A., & Dyer, C. E. (2012). Direct processing of clinically relevant large volume samples for the detection of sexually transmitted infectious agents from urine on a microfluidic device. Analytical Methods, 4(7), 2141-2144. https://doi.org/10.1039/c2ay25075f

• Journal Article Type: Article
• Acceptance Date: May 9, 2012
• Online Publication Date: May 10, 2012
• Publication Date: Jul 1, 2012
• Journal: ANALYTICAL METHODS
• Print ISSN: 1759-9660
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 4
• Issue: 7
• Pages: 2141-2144
• DOI: https://doi.org/10.1039/c2ay25075f
• Keywords: General Engineering; Analytical Chemistry; General Chemical Engineering
• Public URL: https://hull-repository.worktribe.com/output/417821