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Integrated DNA extraction and amplification using electrokinetic pumping in a microfluidic device

Parton, Joseph; Birch, Christopher; Kemp, Cordula; Haswell, Stephen J.; Pamme, Nicole; Shaw, Kirsty J.

Authors

Joseph Parton

Christopher Birch

Cordula Kemp Cordula.Kemp@hull.ac.uk

Stephen J. Haswell

Kirsty J. Shaw



Abstract

An integrated system employing anion exchange for the extraction of DNA from biological samples prior to polymerase chain reaction DNA amplification has been developed, based on microfluidic methodology utilising electrokinetic pumping. In this system, the biological samples were added directly to chitosan-coated silica beads to facilitate DNA immobilisation. The purified, pre-concentrated DNA was then eluted using a combination of electro-osmotic flow enhanced with electrophoretic mobility, which enable DNA to be transported by both mechanisms into the DNA amplification chamber. Through optimisation of the DNA elution conditions, average DNA extraction efficiencies of 69.1% were achievable. Subsequent DNA amplification performed on the microfluidic system demonstrated not only the ability to use electrokinetic movement to integrate the two processes on a single device, but also that the quality and quantity of DNA eluted was suitable for downstream analysis. This work offers an attractive real-world to chip interface and a route to simpler Lab-on-a-Chip technology which eliminates the need for moving parts.

Journal Article Type Article
Publication Date Jan 31, 2012
Journal ANALYTICAL METHODS
Print ISSN 1759-9660
Electronic ISSN 1759-9679
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 4
Issue 1
Pages 96-100
APA6 Citation Parton, J., Birch, C., Kemp, C., Haswell, S. J., Pamme, N., & Shaw, K. J. (2012). Integrated DNA extraction and amplification using electrokinetic pumping in a microfluidic device. Analytical Methods, 4(1), 96-100. https://doi.org/10.1039/c1ay05552f
DOI https://doi.org/10.1039/c1ay05552f
Keywords General Engineering; Analytical Chemistry; General Chemical Engineering
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