Direct processing of clinically relevant large volume samples for the detection of sexually transmitted infectious agents from urine on a microfluidic device

Kemp, Cordula; Birch, Christopher; Shaw, Kirsty J.; Nixon, Gavin J.; Docker, Peter T.; Greenman, John; Dyer, Charlotte; Huggett, Jim F.; Haswell, Stephen J.; Foy, Carole A.; Dyer, Charlotte E.

Abstract

Urine is a preferred specimen for nucleic acid-based detection of sexually transmitted infections (STIs) but represents a challenge for microfluidic devices due to low analyte concentrations. We present an extraction methodology enabling rapid on-chip nucleic acid purification directly from clinically relevant sample volumes up to 1 ml and subsequent PCR amplification detection.

Citation

Kemp, C., Birch, C., Shaw, K. J., Nixon, G. J., Docker, P. T., Greenman, J., …Dyer, C. E. (2012). Direct processing of clinically relevant large volume samples for the detection of sexually transmitted infectious agents from urine on a microfluidic device. Analytical Methods, 4(7), 2141-2144. https://doi.org/10.1039/c2ay25075f

• Journal Article Type: Article
• Acceptance Date: May 9, 2012
• Online Publication Date: May 10, 2012
• Publication Date: Jul 1, 2012
• Journal: ANALYTICAL METHODS
• Print ISSN: 1759-9660
• Electronic ISSN: 1759-9679
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 4
• Issue: 7
• Pages: 2141-2144
• DOI: https://doi.org/10.1039/c2ay25075f
• Keywords: General Engineering; Analytical Chemistry; General Chemical Engineering
• Public URL: https://hull-repository.worktribe.com/output/417821