Cordula Kemp
Direct processing of clinically relevant large volume samples for the detection of sexually transmitted infectious agents from urine on a microfluidic device
Kemp, Cordula; Birch, Christopher; Shaw, Kirsty J.; Nixon, Gavin J.; Docker, Peter T.; Greenman, John; Dyer, Charlotte; Huggett, Jim F.; Haswell, Stephen J.; Foy, Carole A.; Dyer, Charlotte E.
Authors
Christopher Birch
Kirsty J. Shaw
Gavin J. Nixon
Peter T. Docker
Professor John Greenman J.Greenman@hull.ac.uk
Professor of Tumour Immunology
Dr Charlotte Dyer C.E.Dyer@hull.ac.uk
Lecturer in Biomedical Sciences
Jim F. Huggett
Stephen J. Haswell
Carole A. Foy
Dr Charlotte Dyer C.E.Dyer@hull.ac.uk
Lecturer in Biomedical Sciences
Abstract
Urine is a preferred specimen for nucleic acid-based detection of sexually transmitted infections (STIs) but represents a challenge for microfluidic devices due to low analyte concentrations. We present an extraction methodology enabling rapid on-chip nucleic acid purification directly from clinically relevant sample volumes up to 1 ml and subsequent PCR amplification detection.
Citation
Kemp, C., Birch, C., Shaw, K. J., Nixon, G. J., Docker, P. T., Greenman, J., …Dyer, C. E. (2012). Direct processing of clinically relevant large volume samples for the detection of sexually transmitted infectious agents from urine on a microfluidic device. Analytical Methods, 4(7), 2141-2144. https://doi.org/10.1039/c2ay25075f
| Journal Article Type | Article |
|---|---|
| Acceptance Date | May 9, 2012 |
| Online Publication Date | May 10, 2012 |
| Publication Date | Jul 1, 2012 |
| Journal | ANALYTICAL METHODS |
| Print ISSN | 1759-9660 |
| Electronic ISSN | 1759-9679 |
| Publisher | Royal Society of Chemistry |
| Peer Reviewed | Peer Reviewed |
| Volume | 4 |
| Issue | 7 |
| Pages | 2141-2144 |
| DOI | https://doi.org/10.1039/c2ay25075f |
| Keywords | General Engineering; Analytical Chemistry; General Chemical Engineering |
| Public URL | https://hull-repository.worktribe.com/output/417821 |