NanoSOSG : a nanostructured fluorescent probe for detection of intracellular singlet oxygen

Ruiz-González, Rubén; Gulías, Òscar; Ruiz-González, Rubén; Bresolí-Obach, Roger; Gulías, Òscar; Agut, Montserrat; Savoie, Huguette; Boyle, Ross W.; Nonell, Santi; Giuntini, Francesca

Abstract

A biocompatible fluorescent nanoprobe for singlet oxygen (¹O₂) detection in biological systems was designed, synthesized, and characterized, that circumvents many of the limitations of the molecular probe Singlet Oxygen Sensor Green® (SOSG). This widely used commercial singlet oxygen probe was covalently linked to a polyacrylamide nanoparticle core using different architectures to optimize the response to ¹O₂. In contrast to its molecular counterpart, the optimum SOSG-based nanoprobe, which we call NanoSOSG, is readily internalized by E. coli cells and does not interact with bovine serum albumin. Furthermore, the spectral characteristics do not change inside cells, and the probe responds to intracellularly generated ¹O₂ with an increase in fluorescence.

Citation

Ruiz-González, R., Gulías, Ò., Ruiz-González, R., Bresolí-Obach, R., Gulías, Ò., Agut, M., …Giuntini, F. (2017). NanoSOSG : a nanostructured fluorescent probe for detection of intracellular singlet oxygen. Angewandte Chemie, 56(11), 2885-2888. https://doi.org/10.1002/anie.201609050

• Journal Article Type: Article
• Acceptance Date: Nov 11, 2016
• Online Publication Date: Feb 2, 2017
• Publication Date: Mar 6, 2017
• Deposit Date: Feb 17, 2017
• Publicly Available Date: Feb 2, 2017
• Journal: Angewandte chemie international edition
• Print ISSN: 1433-7851
• Electronic ISSN: 1521-3773
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 56
• Issue: 11
• Pages: 2885-2888
• DOI: https://doi.org/10.1002/anie.201609050
• Keywords: Fluorescent probes; Intracellular sensors; Nanoparticles; Optical sensors; Singlet oxygen
• Public URL: https://hull-repository.worktribe.com/output/448510
• Publisher URL: http://onlinelibrary.wiley.com/doi/10.1002/anie.201609050/abstract
• Additional Information: Authors' accepted manuscript of article published in: Angewandte chemie international edition, 2017, v.56, issue 11.