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NanoSOSG : a nanostructured fluorescent probe for detection of intracellular singlet oxygen

Ruiz-González, Rubén; Gulías, Òscar; Ruiz-Gonza?lez, Rube?n; Bresolí-Obach, Roger; Guli?as, Òscar; Agut, Montserrat; Savoie, Huguette; Boyle, Ross W.; Nonell, Santi; Giuntini, Francesca

Authors

Rubén Ruiz-González

Òscar Gulías

Rube?n Ruiz-Gonza?lez

Roger Bresolí-Obach

Òscar Guli?as

Montserrat Agut

Huguette Savoie

Santi Nonell

Francesca Giuntini



Abstract

A biocompatible fluorescent nanoprobe for singlet oxygen (¹O₂) detection in biological systems was designed, synthesized, and characterized, that circumvents many of the limitations of the molecular probe Singlet Oxygen Sensor Green® (SOSG). This widely used commercial singlet oxygen probe was covalently linked to a polyacrylamide nanoparticle core using different architectures to optimize the response to ¹O₂. In contrast to its molecular counterpart, the optimum SOSG-based nanoprobe, which we call NanoSOSG, is readily internalized by E. coli cells and does not interact with bovine serum albumin. Furthermore, the spectral characteristics do not change inside cells, and the probe responds to intracellularly generated ¹O₂ with an increase in fluorescence.

Citation

Ruiz-González, R., Gulías, Ò., Ruiz-González, R., Bresolí-Obach, R., Gulías, Ò., Agut, M., …Giuntini, F. (2017). NanoSOSG : a nanostructured fluorescent probe for detection of intracellular singlet oxygen. Angewandte Chemie, 56(11), 2885-2888. https://doi.org/10.1002/anie.201609050

Journal Article Type Article
Acceptance Date Nov 11, 2016
Online Publication Date Feb 2, 2017
Publication Date Mar 6, 2017
Deposit Date Feb 17, 2017
Publicly Available Date Feb 2, 2017
Journal Angewandte chemie international edition
Print ISSN 1433-7851
Electronic ISSN 1521-3773
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 56
Issue 11
Pages 2885-2888
DOI https://doi.org/10.1002/anie.201609050
Keywords Fluorescent probes; Intracellular sensors; Nanoparticles; Optical sensors; Singlet oxygen
Public URL https://hull-repository.worktribe.com/output/448510
Publisher URL http://onlinelibrary.wiley.com/doi/10.1002/anie.201609050/abstract
Additional Information Authors' accepted manuscript of article published in: Angewandte chemie international edition, 2017, v.56, issue 11.

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