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The effects of exogenous lipid on THP-1 cells : an in vitro model of airway aspiration?

Hayman, Yvette A.; Sadofsky, Laura R.; Williamson, James D.; Hart, Simon P.; Williamson, James; Morice, Alyn H.; Hart, Simon; Morice, Alyn; Sadofsky, Laura; Hayman, Yvette

Authors

Yvette A. Hayman

Dr Laura Sadofsky L.R.Sadofsky@hull.ac.uk
Lecturer in Respiratory Medicine/ Academic lead for postgraduate training in HYMS

James D. Williamson

Simon P. Hart

James Williamson

Alyn H. Morice

Simon Hart

Alyn Morice

Dr Laura Sadofsky L.R.Sadofsky@hull.ac.uk
Lecturer in Respiratory Medicine/ Academic lead for postgraduate training in HYMS

Yvette Hayman



Abstract

Chronic inflammatory diseases of the airways are associated with gastro-oesophageal reflux (GOR) and aspiration events. The observation of lipid laden macrophages (LLM) within the airway may indicate aspiration secondary to GOR. The proposed mechanism, that lipid droplets from undigested or partially digested food are aspirated leading to accumulation in scavenging macrophages led us to hypothesise that an activated population of LLM could interact with other immune cells to induce bronchial inflammation. To test this, we generated an in vitro model using differentiated THP-1 cells which were treated with a high fat liquid feed. Here we show that THP-1 cells can take up lipid from the high fat feed independent of actin polymerisation or CD36 dependent phagocytosis. These cells did not exhibit M1 or M2 polarisation. Gene array analysis confirmed over 8000 genes were upregulated by at least two fold following high fat exposure and IL-8 was the most upregulated gene. Pathway analysis revealed upregulation of genes known to be involved in chronic obstructive pulmonary disease (COPD) pathophysiology. We suggest that aspiration and macrophage phagocytosis may be important mechanisms in the aetiology of diseases such as COPD and cystic fibrosis which are characterised by high levels of IL-8 within the airways.

Journal Article Type Article
Publication Date Mar 20, 2017
Journal ERJ open research
Print ISSN 2312-508X
Electronic ISSN 2312-5098
Publisher European Respiratory Society
Peer Reviewed Peer Reviewed
Volume 3
Issue 1
Article Number 00026-2016
Pages 00026-2016
Institution Citation Hayman, Y. A., Sadofsky, L. R., Williamson, J. D., Hart, S. P., & Morice, A. H. (2017). The effects of exogenous lipid on THP-1 cells : an in vitro model of airway aspiration?. Ers Monograph, 3(1), 00026-2016. https://doi.org/10.1183/23120541.00026-2016
DOI https://doi.org/10.1183/23120541.00026-2016
Keywords Macrophages, Lipid laden macrophages, THP-1 cells, Reflux, COPD
Publisher URL http://openres.ersjournals.com/content/3/1/00026-2016
Copyright Statement Copyright ©ERS 2017
This article is open access and distributed under the terms of the Creative Commons Attribution Non-Commercial Licence 4.0.
Additional Information Copy of article first published in: ERJ open research, 2017, v.3, issue1.

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Copyright Statement
Copyright ©ERS 2017
This article is open access and distributed under the terms of the Creative Commons Attribution Non-Commercial Licence 4.0.







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