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Maternal metabolic stress may affect oviduct gatekeeper function

Jordaens, Lies; van Hoeck, V.; Maillo, Veronica; Gutiérrez-Adán, Alfonso; Marei, Waleed; Vlaeminck, Bruno; Thys, Sofie; Sturmey, Roger; Bols, Peter; Leroy, J. L. M. R.

Authors

Lies Jordaens

V. van Hoeck

Veronica Maillo

Alfonso Gutiérrez-Adán

Waleed Marei

Bruno Vlaeminck

Sofie Thys

Peter Bols

J. L. M. R. Leroy



Abstract

We hypothesized that elevated non-esterified fatty acids (NEFA) modify in vitro bovine oviduct epithelial cell (BOEC) metabolism and barrier function. Hereto, BOECs were studied in a polarized system with 24h-treatments at day 9: 1) CONTROL (0µM NEFA + 0%EtOH), 2) SOLVENT CONTROL (0µM NEFA + 0.45%EtOH), 3) BASAL NEFA (720µM NEFA + 0.45%EtOH in the basal compartment), 4) APICAL NEFA (720µM NEFA + 0.45%EtOH in the apical compartment). FITC-albumin was used for monolayer permeability assessment, and related to Transepithelial Electric Resistance (TER). Fatty acid (FA), glucose, lactate and pyruvate concentrations were measured in spent medium. Intracellular lipid droplets (LD) and FA-uptake were studied using Bodipy 493/503 and immunolabelling of FA-transporters (FAT/CD36, FABP3 and caveolin1). BOEC-mRNA was retrieved for qRT-PCR. Results revealed that APICAL NEFA reduced relative TER-increase (46.85%) during treatment, and increased FITC-albumin flux (27.59%) compared to other treatments. In BASAL NEFA, FAs were transferred to the apical compartment as free FAs: mostly palmitic and oleic acid increased, respectively 56.0 % and 33.5% of initial FA-concentrations. APICAL NEFA allowed no FA-transfer, but induced LD-accumulation and upregulated FA-transporter expression (↑CD36, ↑FABP3, ↑CAV1-protein-expression). Gene expression in APICAL NEFA indicated increased anti-apoptotic (↑BCL2) and anti-oxidative (↑SOD1) capacity, upregulated lipid metabolism (↑CPT1, ↑ACSL1 and ↓ACACA), and FA-uptake (↑CAV1). All treatments had similar carbohydrate metabolism and oviduct function specific gene expression (=OVGP1, ESR1, FOXJ1). Overall, elevated NEFAs affected BOEC-metabolism and barrier function differently depending on NEFA-exposure side. Data substantiate the concept of the oviduct as a gatekeeper that may actively alter early embryonic developmental conditions.

Citation

Jordaens, L., van Hoeck, V., Maillo, V., Gutiérrez-Adán, A., Marei, W., Vlaeminck, B., …Leroy, J. L. M. R. (2017). Maternal metabolic stress may affect oviduct gatekeeper function. Reproduction, 153(6), (759-773). doi:10.1530/rep-16-0569. ISSN 1470-1626

Journal Article Type Article
Acceptance Date Mar 2, 2017
Online Publication Date Mar 3, 2017
Publication Date 2017-06
Deposit Date Mar 3, 2017
Publicly Available Date Mar 13, 2018
Journal Reproduction
Print ISSN 1470-1626
Electronic ISSN 1741-7899
Publisher BioScientifica
Peer Reviewed Peer Reviewed
Volume 153
Issue 6
Pages 759-773
DOI https://doi.org/10.1530/rep-16-0569
Keywords NEFAs; Oviduct; TER, Fatty acid transfer; Metabolism
Public URL https://hull-repository.worktribe.com/output/449154
Publisher URL http://www.reproduction-online.org/content/early/2017/03/03/REP-16-0569.abstract
Copyright Statement ©2018 University of Hull
Additional Information Disclaimer: this is not the definitive version of record of this article.This manuscript has been accepted for publication in Reproduction, but the version presented here has not yet been copy-edited, formatted or proofed. Consequently, Bioscientifica accepts no responsibility for any errors or omissions it may contain. The definitive version is now freely available at http://dx.doi.org/10.1530/REP-16-0569 (2017)

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