Maternal metabolic stress may affect oviduct gatekeeper function

Jordaens, Lies; van Hoeck, V.; Maillo, Veronica; Gutie?rrez-Ada?n, Alfonso; Marei, Waleed; Vlaeminck, Bruno; Thys, Sofie; Sturmey, Roger; Bols, Peter; Leroy, J. L. M. R.

Abstract

We hypothesized that elevated non-esterified fatty acids (NEFA) modify in vitro bovine oviduct epithelial cell (BOEC) metabolism and barrier function. Hereto, BOECs were studied in a polarized system with 24h-treatments at day 9: 1) CONTROL (0µM NEFA + 0%EtOH), 2) SOLVENT CONTROL (0µM NEFA + 0.45%EtOH), 3) BASAL NEFA (720µM NEFA + 0.45%EtOH in the basal compartment), 4) APICAL NEFA (720µM NEFA + 0.45%EtOH in the apical compartment). FITC-albumin was used for monolayer permeability assessment, and related to Transepithelial Electric Resistance (TER). Fatty acid (FA), glucose, lactate and pyruvate concentrations were measured in spent medium. Intracellular lipid droplets (LD) and FA-uptake were studied using Bodipy 493/503 and immunolabelling of FA-transporters (FAT/CD36, FABP3 and caveolin1). BOEC-mRNA was retrieved for qRT-PCR. Results revealed that APICAL NEFA reduced relative TER-increase (46.85%) during treatment, and increased FITC-albumin flux (27.59%) compared to other treatments. In BASAL NEFA, FAs were transferred to the apical compartment as free FAs: mostly palmitic and oleic acid increased, respectively 56.0 % and 33.5% of initial FA-concentrations. APICAL NEFA allowed no FA-transfer, but induced LD-accumulation and upregulated FA-transporter expression (↑CD36, ↑FABP3, ↑CAV1-protein-expression). Gene expression in APICAL NEFA indicated increased anti-apoptotic (↑BCL2) and anti-oxidative (↑SOD1) capacity, upregulated lipid metabolism (↑CPT1, ↑ACSL1 and ↓ACACA), and FA-uptake (↑CAV1). All treatments had similar carbohydrate metabolism and oviduct function specific gene expression (=OVGP1, ESR1, FOXJ1). Overall, elevated NEFAs affected BOEC-metabolism and barrier function differently depending on NEFA-exposure side. Data substantiate the concept of the oviduct as a gatekeeper that may actively alter early embryonic developmental conditions.

Citation

Jordaens, L., van Hoeck, V., Maillo, V., Gutiérrez-Adán, A., Marei, W., Vlaeminck, B., …Leroy, J. L. M. R. (2017). Maternal metabolic stress may affect oviduct gatekeeper function. Reproduction, 153(6), 759-773. https://doi.org/10.1530/rep-16-0569

• Journal Article Type: Article
• Acceptance Date: Mar 2, 2017
• Online Publication Date: Mar 3, 2017
• Publication Date: 2017-06
• Deposit Date: Mar 3, 2017
• Publicly Available Date: Mar 13, 2018
• Journal: Reproduction
• Print ISSN: 1470-1626
• Electronic ISSN: 1741-7899
• Publisher: BioScientifica
• Peer Reviewed: Peer Reviewed
• Volume: 153
• Issue: 6
• Pages: 759-773
• DOI: https://doi.org/10.1530/rep-16-0569
• Keywords: NEFAs; Oviduct; TER, Fatty acid transfer; Metabolism
• Public URL: https://hull-repository.worktribe.com/output/449154
• Publisher URL: http://www.reproduction-online.org/content/early/2017/03/03/REP-16-0569.abstract
• Additional Information: Disclaimer: this is not the definitive version of record of this article.This manuscript has been accepted for publication in Reproduction, but the version presented here has not yet been copy-edited, formatted or proofed. Consequently, Bioscientifica accepts no responsibility for any errors or omissions it may contain. The definitive version is now freely available at http://dx.doi.org/10.1530/REP-16-0569 (2017)