Vitamin D3 supplementation of a high fat high sugar diet ameliorates prediabetic phenotype in female LDLR–/–and LDLR+/+mice

Carroll, Sean; Hobkirk, James; Browning, Michael J.; Janus, Justyna; Kheder, Ramiar; Saeed, Zeayd; Stover, Cordula

Abstract

© 2017 The Authors. Immunity, Inflammation and Disease Published by John Wiley & Sons Ltd. INTRODUCTION: Fatty liver disease is prevalent in populations with high caloric intake. Nutritherapeutic approaches are being considered, such as supplementary Vitamin D 3 , to improve aspects of metabolic syndrome, namely fatty liver disease, hyperlipidemia, and insulin resistance associated with obesity. METHODS: We analyzed female LDLR -/- and LDLR +/+ mice on a 10-week diabetogenic diet for markers of fatty liver disease, metabolic strain, and inflammation. RESULTS: The groups on a high fat high sugar diet with supplementary Vitamin D 3 , in comparison with the groups on a high fat high sugar diet alone, showed improved transaminase levels, significantly less hypertriglyceridemia and hyperinsulinemia, and histologically, there was less pericentral hepatic steatosis. Levels of non-esterified fatty acids and lipid peroxidation products were significantly lower in the group supplemented with additional Vitamin D 3 , as were systemic markers of inflammation (serum endotoxin and IL-6). M2 macrophage phenotype predominated in the group supplemented with additional Vitamin D 3 . Beneficial changes were observed as early as five weeks’ supplementation with Vitamin D 3 and extended to restoration of high fat high sugar diet induced decrease of bone mineral density. CONCLUSION: In summary, Vitamin D 3 was a significantly beneficial dietary additive to blunt a prediabetic phenotype in diet-induced obesity of female LDLR -/- and LDLR +/+ mice.

Citation

Carroll, S., Hobkirk, J., Browning, M. J., Janus, J., Kheder, R., Saeed, Z., & Stover, C. (2017). Vitamin D3 supplementation of a high fat high sugar diet ameliorates prediabetic phenotype in female LDLR–/–and LDLR+/+mice. Immunity, Inflammation and Disease, 5(2), 151-154. https://doi.org/10.1002/iid3.154

• Acceptance Date: Jan 31, 2017
• Online Publication Date: Mar 13, 2017
• Publication Date: 2017-06
• Deposit Date: Apr 12, 2017
• Publicly Available Date: Apr 12, 2017
• Journal: Immunity, inflammation and disease
• Electronic ISSN: 2050-4527
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 5
• Issue: 2
• Pages: 151-154
• DOI: https://doi.org/10.1002/iid3.154
• Keywords: Diet; Metabolic syndrome; Mouse study; Vitamin D
• Public URL: https://hull-repository.worktribe.com/output/450554
• Publisher URL: http://onlinelibrary.wiley.com/doi/10.1002/iid3.154/abstract?
• Additional Information: Copy of article first published in: Immunity, inflammation and disease, 2017.
• Contract Date: Apr 12, 2017