Dictyostelium Sun-1 connects the centrosome to chromatin and ensures genome stability

Rivero, Francisco; Xiong, Huajiang; Rivero-Crespo, Francisco; Euteneuer, Ursula; Mondal, Subhanjan; Mana-Capelli, Sebastian; Larochelle, Denis; Vogel, Annette; Gassen, Berthold; Noegel, Angelika A.

Abstract

The centrosome-nucleus attachment is a prerequisite for faithful chromosome segregation during mitosis. We addressed the function of the nuclear envelope (NE) protein Sun-1 in centrosome-nucleus connection and the maintenance of genome stability in Dictyostelium discoideum. We provide evidence that Sun-1 requires direct chromatin binding for its inner nuclear membrane targeting. Truncation of the cryptic N-terminal chromatin-binding domain of Sun-1 induces dramatic separation of the inner from the outer nuclear membrane and deformations in nuclear morphology, which are also observed using a Sun-1 RNAi construct. Thus, chromatin binding of Sun-1 defines the integrity of the nuclear architecture. In addition to its role as a NE scaffold, we find that abrogation of the chromatin binding of Sun-1 dissociates the centrosome-nucleus connection, demonstrating that Sun-1 provides an essential link between the chromatin and the centrosome. Moreover, loss of the centrosome-nucleus connection causes severe centrosome hyperamplification and defective spindle formation, which enhances aneuploidy and cell death significantly. We highlight an important new aspect for Sun-1 in coupling the centrosome and nuclear division during mitosis to ensure faithful chromosome segregation

Citation

Rivero, F., Xiong, H., Rivero-Crespo, F., Euteneuer, U., Mondal, S., Mana-Capelli, S., Larochelle, D., Vogel, A., Gassen, B., & Noegel, A. A. (2008). Dictyostelium Sun-1 connects the centrosome to chromatin and ensures genome stability. Traffic, 9(5), 708-724. https://doi.org/10.1111/j.1600-0854.2008.00721.x

• Journal Article Type: Article
• Acceptance Date: Feb 7, 2008
• Online Publication Date: Mar 12, 2008
• Publication Date: May 1, 2008
• Deposit Date: Nov 13, 2014
• Journal: Traffic
• Print ISSN: 1398-9219
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 9
• Issue: 5
• Pages: 708-724
• DOI: https://doi.org/10.1111/j.1600-0854.2008.00721.x
• Keywords: Aneuploidy, Cell Death, Chromatin, Dictyostelium, Genome, Role,
• Public URL: https://hull-repository.worktribe.com/output/465036
• Contract Date: Nov 23, 2017