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The effect of hyperbaric oxygen preconditioning on heat shock protein 72 expression following in vitro stress in human monocytes

Vince, Rebecca V.; Midgley, Adrian W.; Laden, Gerard; Madden, Leigh A.

Authors

Adrian W. Midgley

Gerard Laden



Abstract

Hyperbaric oxygen (HBO) is thought to confer protection to cells via a cellular response to free radicals. This process may involve increased expression of heat shock proteins, in particular the highly inducible heat shock protein 72 (Hsp72). Healthy male volunteers (n = 16) were subjected to HBO for 1h at 2.8ATA. Inducible Hsp72 expression was measured by flow cytometry pre-, post- and 4h-post HBO. Peripheral blood mononuclear cells (PBMC) were isolated from whole blood via density centrifugation pre-, post- and 4h post-HBO. PBMC were then subjected to an in vitro heat shock at 40°C or hypoxia at 37°C (5% O(2)) with a control at 37°C. Cells were then analysed for Hsp72 expression by flow cytometry. Monocytes showed no significant changes in Hsp72 expression following HBO. No detectable Hsp72 was seen in lymphocytes or neutrophils. Following in vitro hypoxic exposure, a significant increase in Hsp72 expression was observed in monocytes isolated immediately post- (p = 0.006) and 4h post-HBO (p = 0.010) in comparison to control values. HBO does not induce Hsp72 expression in PBMC. The reported benefits of HBO in terms of pre-conditioning are not due to inducement of Hsp72 expression in circulating blood cells, but may involve an enhancement of the stress response.

Citation

Vince, R. V., Midgley, A. W., Laden, G., & Madden, L. A. (2011). The effect of hyperbaric oxygen preconditioning on heat shock protein 72 expression following in vitro stress in human monocytes. Cell Stress and Chaperones, 16(3), 339-343. https://doi.org/10.1007/s12192-010-0246-2

Journal Article Type Article
Acceptance Date Feb 1, 2010
Online Publication Date Dec 5, 2010
Publication Date 2011-05
Deposit Date Nov 13, 2014
Publicly Available Date Nov 13, 2014
Journal Cell Stress & Chaperones
Print ISSN 1355-8145
Electronic ISSN 1466-1268
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 16
Issue 3
Pages 339-343
DOI https://doi.org/10.1007/s12192-010-0246-2
Keywords Hyperbaric oxygen, Heat shock, Hypoxia, Monocytes.
Public URL https://hull-repository.worktribe.com/output/465801

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