The CD105:CD106 microparticle ratio is CD106 dominant in polycystic ovary syndrome compared to type 2 diabetes and healthy subjects

Al-Qaissi, Ahmed; Alqarni, Saeed; Javed, Zeeshan; Atkin, Stephen L.; Sathyapalan, Thozhukat; Vince, Rebecca; Madden, Leigh A.

Abstract

© 2019, The Author(s). Background: A retrospective analysis was carried out from patients and controls during the past 5 years from a series of studies investigating endothelial microparticles (MP). Methods: In total, 319 samples from 207 individuals were included in this analysis, from patients with type 2 diabetes (T2D, n = 105), women with polycystic ovary syndrome (PCOS, n = 145) and healthy volunteers (n = 69). All data were generated via the same flow cytometry protocol with the same antibody clones. Endothelial markers CD105 (Endoglin) and CD106 (Vascular cell adhesion molecule-1) were used to enumerate MP in venous blood. Results: The ratio of CD105MP:CD106MP was significantly different between groups (F = 63.43, p < 0.0001). Women with PCOS were found to have a median CD105MP:CD106MP ratio of 0.40 (IQR 0.24–0.57), suggesting approximately two CD106MP were found per CD105MP. The T2D group showed a median ratio of 2.32 (1.51–3.69) whereas in healthy volunteers the ratio was 2.21 (1.63–3.55). Serum intercellular adhesion molecule-1 was also shown to be significantly increased in PCOS when compared with control or T2D groups (F = 14.5, p < 0.001). Conclusion: These data suggest that women with PCOS have an altered endothelial MP release in favour of CD106. Thus a potential activated endothelial state exists in women with PCOS with a shift towards a predominantly CD106MP profile.

Citation

Al-Qaissi, A., Alqarni, S., Javed, Z., Atkin, S. L., Sathyapalan, T., Vince, R., & Madden, L. A. (2019). The CD105:CD106 microparticle ratio is CD106 dominant in polycystic ovary syndrome compared to type 2 diabetes and healthy subjects. Endocrine, https://doi.org/10.1007/s12020-019-02059-9

• Journal Article Type: Article
• Acceptance Date: Aug 12, 2019
• Online Publication Date: Aug 27, 2019
• Publication Date: Aug 27, 2019
• Deposit Date: Aug 31, 2019
• Publicly Available Date: Sep 2, 2019
• Journal: Endocrine
• Print ISSN: 1355-008X
• Electronic ISSN: 1559-0100
• Publisher: Springer (part of Springer Nature)
• Peer Reviewed: Peer Reviewed
• DOI: https://doi.org/10.1007/s12020-019-02059-9
• Keywords: Polycystic ovary syndrome; Endothelial microparticles; VCAM-1
• Public URL: https://hull-repository.worktribe.com/output/2538616
• Publisher URL: https://link.springer.com/article/10.1007%2Fs12020-019-02059-9