Hypoxia mediated release of endothelial microparticles and increased association of S100A12 with circulating neutrophils

Vince, Rebecca V.; Chrismas, Bryna; Midgley, Adrian W.; McNaughton, Lars R.; Madden, Leigh A.

Abstract

Microparticles are released from the endothelium under normal homeostatic conditions and have been shown elevated in disease states, most notably those characterised by endothelial dysfunction. The endothelium is sensitive to oxidative stress/status and vascular cell adhesion molecule-1 (VCAM-1) expression is upregulated upon activated endothelium, furthermore the presence of VCAM-1 on microparticles is known. S100A12, a calcium binding protein part of the S100 family, is shown to be present on circulating leukocytes and is thought a sensitive marker to local inflammatory process, which may be driven by oxidative stress. Eight healthy males were subjected to breathing hypoxic air (15% O 2 , approximately equivalent to 3000 metres altitude) for 80 minutes in a temperature controlled laboratory and venous blood samples were processed immediately for VCAM-1 microparticles (VCAM-1 MP) and S100A12 association with leukocytes by flow cytometry. A pre-hypoxic blood sample was used for comparison. Both VCAM-1 MP and S100A12 association with neutrophils were significantly elevated post hypoxic breathing later declining to levels observed in the pre-test samples. A similar trend was observed in both cases and a correlation may exist between these two markers in response to hypoxia. These data offer evidence using novel markers of endothelial and circulating blood responses to hypoxia. ©2009 Landes Bioscience.

Citation

Vince, R. V., Chrismas, B., Midgley, A. W., McNaughton, L. R., & Madden, L. A. (2009). Hypoxia mediated release of endothelial microparticles and increased association of S100A12 with circulating neutrophils. Oxidative Medicine and Cellular Longevity, 2(1), 2-6. https://doi.org/10.4161/oxim.2.1.7611

• Acceptance Date: Dec 11, 2008
• Publication Date: 2009
• Deposit Date: Nov 13, 2014
• Publicly Available Date: Nov 13, 2014
• Journal: Oxidative medicine and cellular longevity
• Print ISSN: 1942-0900
• Electronic ISSN: 1942-0994
• Publisher: Hindawi
• Peer Reviewed: Peer Reviewed
• Volume: 2
• Issue: 1
• Pages: 2-6
• DOI: https://doi.org/10.4161/oxim.2.1.7611
• Keywords: Cell Biology; Biochemistry; Ageing; General Medicine
• Public URL: https://hull-repository.worktribe.com/output/472878
• Publisher URL: https://www.hindawi.com/journals/omcl/2009/137280/abs/
• Additional Information: Copy of article first published in: Oxidative medicine and cellular longevity, 2009, v.2, issue 1.