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Hypoxia mediated release of endothelial microparticles and increased association of S100A12 with circulating neutrophils

Vince, Rebecca V.; Chrismas, Bryna; Midgley, Adrian W.; McNaughton, Lars R.; Madden, Leigh A.

Authors

Bryna Chrismas

Adrian W. Midgley

Lars R. McNaughton

Leigh A. Madden

Abstract

Microparticles are released from the endothelium under normal homeostatic conditions and have been shown elevated in disease states, most notably those characterised by endothelial dysfunction. The endothelium is sensitive to oxidative stress/status and vascular cell adhesion molecule-1 (VCAM-1) expression is upregulated upon activated endothelium, furthermore the presence of VCAM-1 on microparticles is known. S100A12, a calcium binding protein part of the S100 family, is shown to be present on circulating leukocytes and is thought a sensitive marker to local inflammatory process, which may be driven by oxidative stress. Eight healthy males were subjected to breathing hypoxic air (15% O 2 , approximately equivalent to 3000 metres altitude) for 80 minutes in a temperature controlled laboratory and venous blood samples were processed immediately for VCAM-1 microparticles (VCAM-1 MP) and S100A12 association with leukocytes by flow cytometry. A pre-hypoxic blood sample was used for comparison. Both VCAM-1 MP and S100A12 association with neutrophils were significantly elevated post hypoxic breathing later declining to levels observed in the pre-test samples. A similar trend was observed in both cases and a correlation may exist between these two markers in response to hypoxia. These data offer evidence using novel markers of endothelial and circulating blood responses to hypoxia. ©2009 Landes Bioscience.

Journal Article Type Article
Publication Date 2009
Journal Oxidative medicine and cellular longevity
Print ISSN 1942-0900
Electronic ISSN 1942-0994
Publisher Hindawi Publishing Corporation
Peer Reviewed Peer Reviewed
Volume 2
Issue 1
Pages 2-6
DOI https://doi.org/10.4161/oxim.2.1.7611
Keywords Cell Biology; Biochemistry; Ageing; General Medicine
Publisher URL https://www.hindawi.com/journals/omcl/2009/137280/abs/
Copyright Statement © 2009 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Additional Information Copy of article first published in: Oxidative medicine and cellular longevity, 2009, v.2, issue 1.

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Copyright Statement
© 2009 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.



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