Skip to main content

Research Repository

Advanced Search

On-chip magnetic particle-based immunoassays using multilaminar flow for clinical diagnostics

Tarn, Mark D.; Pamme, Nicole

Authors

Mark D. Tarn

Nicole Pamme



Contributors

V. Taly
Editor

J. L. Viovy
Editor

S. Descroix
Editor

Abstract

Magnetic particles have become popular in recent years for immunoassays due to their high surface-to-volume ratio and the ease of their manipulation. However, such assays also require multiple reaction and washing steps that are both time-consuming and manually laborious. Here, we describe a setup and methodology for performing rapid immunoassays on magnetic particles in continuous flow via their deflection through multiple laminar flow streams of reagents and washing solutions. In particular, we focus on the use of the microfluidic platform for a C-reactive protein (CRP) sandwich immunoassay in less than 60 s.

Citation

Tarn, M. D., & Pamme, N. (2017). On-chip magnetic particle-based immunoassays using multilaminar flow for clinical diagnostics. In V. Taly, J. L. Viovy, & S. Descroix (Eds.), Microchip Diagnostics : Methods and Protocols (69-83). New York: Humana Press. https://doi.org/10.1007/978-1-4939-6734-6_6

Online Publication Date Jan 3, 2017
Publication Date Jan 1, 2017
Deposit Date Dec 8, 2020
Publisher Humana Press
Volume 1547
Pages 69-83
Series Title Methods in Molecular Biology
Series Number 1547
Series ISSN 1064-3745 1940-6029
Book Title Microchip Diagnostics : Methods and Protocols
ISBN 9781493967322
DOI https://doi.org/10.1007/978-1-4939-6734-6_6
Keywords Continuous flow; Deflection Immunoassays; Microfluidics; Microparticles; Multilaminar flow
Public URL https://hull-repository.worktribe.com/output/519788