On-chip magnetic particle-based immunoassays using multilaminar flow for clinical diagnostics

Tarn, Mark D.; Pamme, Nicole

Abstract

Magnetic particles have become popular in recent years for immunoassays due to their high surface-to-volume ratio and the ease of their manipulation. However, such assays also require multiple reaction and washing steps that are both time-consuming and manually laborious. Here, we describe a setup and methodology for performing rapid immunoassays on magnetic particles in continuous flow via their deflection through multiple laminar flow streams of reagents and washing solutions. In particular, we focus on the use of the microfluidic platform for a C-reactive protein (CRP) sandwich immunoassay in less than 60 s.

Citation

Tarn, M. D., & Pamme, N. (2017). On-chip magnetic particle-based immunoassays using multilaminar flow for clinical diagnostics. In V. Taly, J. L. Viovy, & S. Descroix (Eds.), Microchip Diagnostics : Methods and Protocols (69-83). New York: Humana Press. https://doi.org/10.1007/978-1-4939-6734-6_6

• Online Publication Date: Jan 3, 2017
• Publication Date: Jan 1, 2017
• Deposit Date: Dec 8, 2020
• Publisher: Humana Press
• Volume: 1547
• Pages: 69-83
• Series Title: Methods in Molecular Biology
• Series Number: 1547
• Series ISSN: 1064-3745 1940-6029
• Book Title: Microchip Diagnostics : Methods and Protocols
• ISBN: 9781493967322
• DOI: https://doi.org/10.1007/978-1-4939-6734-6_6
• Keywords: Continuous flow; Deflection Immunoassays; Microfluidics; Microparticles; Multilaminar flow
• Public URL: https://hull-repository.worktribe.com/output/519788