Ectopic expression of cyclase associated protein CAP restores the streaming and aggregation defects of adenylyl cyclase a deficient Dictyostelium discoideum cells

Sultana, Hameeda; Neelakanta, Girish; Rivero, Francisco; Blau-Wasser, Rosemarie; Schleicher, Michael; Noegel, Angelika A.

Abstract

Background: Cell adhesion, an integral part of D. discoideum development, is important for morphogenesis and regulated gene expression in the multicellular context and is required to trigger cell-differentiation. G-protein linked adenylyl cyclase pathways are crucially involved and a mutant lacking the aggregation specific adenylyl cyclase ACA does not undergo multicellular development. Results: Here, we have investigated the role of cyclase-associated protein (CAP), an important regulator of cell polarity and F-actin/G-actin ratio in the aca - mutant. We show that ectopic expression of GFP-CAP improves cell polarization, streaming and aggregation in aca - cells, but it fails to completely restore development. Our studies indicate a requirement of CAP in the ACA dependent signal transduction for progression of the development of unicellular amoebae into multicellular structures. The reduced expression of the cell adhesion molecule DdCAD1 together with csA is responsible for the defects in aca - cells to initiate multicellular development. Early development was restored by the expression of GFP-CAP that enhanced the DdCAD1 transcript levels and to a lesser extent the csA mRNA levels. Conclusions: Collectively, our data shows a novel role of CAP in regulating cell adhesion mechanisms during development that might be envisioned to unravel the functions of mammalian CAP during animal embryogenesis. © 2012 Sultana et al; licensee BioMed Central Ltd.

Citation

Sultana, H., Neelakanta, G., Rivero, F., Blau-Wasser, R., Schleicher, M., & Noegel, A. A. (2012). Ectopic expression of cyclase associated protein CAP restores the streaming and aggregation defects of adenylyl cyclase a deficient Dictyostelium discoideum cells. BMC developmental biology, 12, Article 3. https://doi.org/10.1186/1471-213X-12-3

• Journal Article Type: Article
• Acceptance Date: Jan 12, 2012
• Online Publication Date: Jan 12, 2012
• Publication Date: Jan 13, 2012
• Deposit Date: Apr 1, 2022
• Publicly Available Date: Apr 20, 2022
• Journal: BMC Developmental Biology
• Electronic ISSN: 1471-213X
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 12
• Article Number: 3
• DOI: https://doi.org/10.1186/1471-213X-12-3
• Keywords: Cell polarity; Aggregation; Cell-adhesion; Development; Cyclase associated protein; Adenylyl cyclase ACA; Adenylyl cyclase ACB
• Public URL: https://hull-repository.worktribe.com/output/540952