Pulsed extremely low-frequency magnetic fields stimulate microvesicle release from human monocytic leukaemia cells

Stratton, Dan; Lange, Sigrun; Inal, Jameel M.

Abstract

Microvesicles are released from cell surfaces constitutively during early apoptosis or upon activation with various stimuli including sublytic membrane attack complex (MAC). This study shows that an alternating current, pulsed, extremely low-frequency electromagnetic field (0.3 μT at 10. Hz, 6. V AC) induced transient plasma membrane damage that allowed calcium influx. This in turn caused a release of stimulated microvesicles (sMV). When extracellular calcium was chelated with EGTA, sMV biogenesis initiated by ELFMF was markedly reduced and the reduction was less than when the stimulation was the deposition of sublytic MAC. This suggested that pulsed ELFMF resulted in transcellular membrane pores causing organelles to leak additional calcium into the cytoplasm (which EGTA would not chelate) which itself can lead to sMV release. © 2012 Elsevier Inc.

Citation

Stratton, D., Lange, S., & Inal, J. M. (2013). Pulsed extremely low-frequency magnetic fields stimulate microvesicle release from human monocytic leukaemia cells. Biochemical and biophysical research communications, 430(2), 470-475. https://doi.org/10.1016/j.bbrc.2012.12.012

• Journal Article Type: Article
• Acceptance Date: Nov 29, 2012
• Online Publication Date: Dec 10, 2012
• Publication Date: Jan 11, 2013
• Deposit Date: Jul 4, 2018
• Journal: Biochemical and Biophysical Research Communications
• Print ISSN: 0006-291X
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 430
• Issue: 2
• Pages: 470-475
• DOI: https://doi.org/10.1016/j.bbrc.2012.12.012
• Keywords: Extremely low-frequency magnetic field; Stimulated microvesicle; Calcium homeostasis; Plasma membrane
• Public URL: https://hull-repository.worktribe.com/output/910317
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0006291X12023406?via%3Dihub