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Pulsed extremely low-frequency magnetic fields stimulate microvesicle release from human monocytic leukaemia cells

Stratton, Dan; Lange, Sigrun; Inal, Jameel M.

Authors

Sigrun Lange

Jameel M. Inal



Abstract

Microvesicles are released from cell surfaces constitutively during early apoptosis or upon activation with various stimuli including sublytic membrane attack complex (MAC). This study shows that an alternating current, pulsed, extremely low-frequency electromagnetic field (0.3 μT at 10. Hz, 6. V AC) induced transient plasma membrane damage that allowed calcium influx. This in turn caused a release of stimulated microvesicles (sMV). When extracellular calcium was chelated with EGTA, sMV biogenesis initiated by ELFMF was markedly reduced and the reduction was less than when the stimulation was the deposition of sublytic MAC. This suggested that pulsed ELFMF resulted in transcellular membrane pores causing organelles to leak additional calcium into the cytoplasm (which EGTA would not chelate) which itself can lead to sMV release. © 2012 Elsevier Inc.

Journal Article Type Article
Publication Date Jan 11, 2013
Journal Biochemical and Biophysical Research Communications
Print ISSN 0006-291X
Electronic ISSN 1090-2104
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 430
Issue 2
Pages 470-475
APA6 Citation Stratton, D., Lange, S., & Inal, J. M. (2013). Pulsed extremely low-frequency magnetic fields stimulate microvesicle release from human monocytic leukaemia cells. Biochemical and biophysical research communications, 430(2), 470-475. doi:10.1016/j.bbrc.2012.12.012
DOI https://doi.org/10.1016/j.bbrc.2012.12.012
Keywords Extremely low-frequency magnetic field; Stimulated microvesicle; Calcium homeostasis; Plasma membrane
Publisher URL https://www.sciencedirect.com/science/article/pii/S0006291X12023406?via%3Dihub
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