Microvesicles released constitutively from prostate cancer cells differ biochemically and functionally to stimulated microvesicles released through sublytic C5b-9

Stratton, Dan; Moore, Colin; Antwi-Baffour, Samuel; Lange, Sigrun; Inal, Jameel

Abstract

© 2015 Elsevier Inc. All rights reserved. We have classified microvesicles into two subtypes: larger MVs released upon stimulation of prostate cancer cells, sMVs, and smaller cMVs, released constitutively. cMVs are released as part of cell metabolism and sMVs, released at 10-fold higher levels, produced upon activation, including sublytic C5b-9. From electron microscopy, nanosight tracking analysis, dynamic light scattering and flow cytometry, cMVs (194-210 nm in diameter) are smaller than sMVs (333-385 nm). Furthermore, using a Quartz Crystal Microbalance measuring changes in resonant frequency (Δf) that equate to mass deposited on a sensor, an sMV and a cMV are estimated at 0.267 and 0.241 pg, respectively. sMVs carry more calcium and protein, express higher levels of lipid rafts, GPI-anchored CD55 and phosphatidylserine including deposited C5b-9 compared to cMVs. This may allude to biological differences such as increased bound C4BP on sMVs inhibiting complement more effectively.

Citation

Stratton, D., Moore, C., Antwi-Baffour, S., Lange, S., & Inal, J. (2015). Microvesicles released constitutively from prostate cancer cells differ biochemically and functionally to stimulated microvesicles released through sublytic C5b-9. Biochemical and biophysical research communications, 460(3), 589-595. https://doi.org/10.1016/j.bbrc.2015.03.074

• Journal Article Type: Article
• Acceptance Date: Mar 24, 2015
• Publication Date: May 8, 2015
• Deposit Date: Jul 4, 2018
• Journal: Biochemical and Biophysical Research Communications
• Print ISSN: 0006-291X
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 460
• Issue: 3
• Pages: 589-595
• DOI: https://doi.org/10.1016/j.bbrc.2015.03.074
• Keywords: Constitutive microvesicles; Stimulated microvesicles; Complement inhibition
• Public URL: https://hull-repository.worktribe.com/output/910391
• Related Public URLs: http://uhra.herts.ac.uk/handle/2299/19607