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Comparative PBT screening using (Q)SAR tools within REACH legislation (2009)
Journal Article
Zachary, M., & Greenway, G. M. (2009). Comparative PBT screening using (Q)SAR tools within REACH legislation. SAR and QSAR in Environmental Research, 20(1-2), 145-157. https://doi.org/10.1080/10629360902724143

Small to medium sized enterprises (SMEs) in the EU are facing challenges due to the introduction of new legislation designed to protect consumers and the environment, REACH (Registration, Evaluation, Authorisation and Restriction of CHemicals). There... Read More about Comparative PBT screening using (Q)SAR tools within REACH legislation.

Development of a high throughput screening tool for biotransformations utilising a thermophilic L-aminoacylase enzyme (2009)
Journal Article
Ngamsom, B., Hickey, A. M., Greenway, G. M., Littlechild, J. A., Watts, P., & Wiles, C. (2010). Development of a high throughput screening tool for biotransformations utilising a thermophilic L-aminoacylase enzyme. Journal of Molecular Catalysis B: Enzymatic, 63(1-2), 81-86. https://doi.org/10.1016/j.molcatb.2009.12.013

Micro-reactors containing a monolith-immobilised thermophilic l-aminoacylase, from Thermococcus litoralis, have been developed for use in biotransformation reactions and a study has been carried out to investigate the stereospecificity and stability... Read More about Development of a high throughput screening tool for biotransformations utilising a thermophilic L-aminoacylase enzyme.

Development of a monolith based immobilized lipase micro-reactor for biocatalytic reactions in a biphasic mobile system (2009)
Journal Article
He, P., Greenway, G., & Haswell, S. J. (2010). Development of a monolith based immobilized lipase micro-reactor for biocatalytic reactions in a biphasic mobile system. Process Biochemistry, 45(4), 593-597. https://doi.org/10.1016/j.procbio.2009.12.008

This paper reports a simple method for producing macroporous silica-monoliths with controllable porosity that can be used for the immobilization of lipases to generate an active and stable micro-reactor for biocatalysis. A range of commercially avail... Read More about Development of a monolith based immobilized lipase micro-reactor for biocatalytic reactions in a biphasic mobile system.

Measurement of acetylcholinesterase inhibition using bienzymes immobilized monolith micro-reactor with integrated electrochemical detection (2009)
Journal Article
He, P., Davies, J., Greenway, G., & Haswell, S. J. (2010). Measurement of acetylcholinesterase inhibition using bienzymes immobilized monolith micro-reactor with integrated electrochemical detection. Analytica Chimica Acta, 659(1-2), 9-14. https://doi.org/10.1016/j.aca.2009.11.052

This paper reports a simple μ-FIA based method for the rapid evaluation of acetylcholinesterase inhibition based on bienzymes immobilized monolith micro-reactor, with integrated electrochemical detection. The monolith was prepared inside a micro-flui... Read More about Measurement of acetylcholinesterase inhibition using bienzymes immobilized monolith micro-reactor with integrated electrochemical detection.

PDMS Microfluidic Device with an Integrated Optical Sensor for Determination of Zinc (II) in Pharmaceuticals: Toward the Production of Miniaturized Green Chemistry Analytical Systems (2009)
Journal Article
AlSuhaimi, A., Greenway, G. M., McCreedy, T., & Youngvises, N. (in press). PDMS Microfluidic Device with an Integrated Optical Sensor for Determination of Zinc (II) in Pharmaceuticals: Toward the Production of Miniaturized Green Chemistry Analytical Systems. Spectroscopy letters, 42(6-7), 370-375. https://doi.org/10.1080/00387010903187260

A simple miniaturized system was fabricated in general laboratory from polydimethylsiloxane (PDMS) according to a prototype casting and molding process permitting the construction of three-dimensional (3D) microchannel (100 mu m i.d.) with T-type net... Read More about PDMS Microfluidic Device with an Integrated Optical Sensor for Determination of Zinc (II) in Pharmaceuticals: Toward the Production of Miniaturized Green Chemistry Analytical Systems.

Development of environmental water monitors based on hydrostatic and flourescence detection techniques (2009)
Thesis
Scott, R. M. (2009). Development of environmental water monitors based on hydrostatic and flourescence detection techniques. (Thesis). University of Hull. Retrieved from https://hull-repository.worktribe.com/output/4209333

The research for this thesis led to the development of two environmental detectors, whose function was to identify predetermined threshold levels of the analyte. The research centred onto two types of analyte; immiscible and dissolved, both occurring... Read More about Development of environmental water monitors based on hydrostatic and flourescence detection techniques.

Development of a gel-to-gel electro-kinetic pinched injection method for an integrated micro-fluidic based DNA analyser (2009)
Journal Article
Oakley, J. A., Robinson, S., Dyer, C. E., Greenman, J., Greenway, G. M., & Haswell, S. J. (2009). Development of a gel-to-gel electro-kinetic pinched injection method for an integrated micro-fluidic based DNA analyser. Analytica Chimica Acta, 652(1-2), 239-244. https://doi.org/10.1016/j.aca.2009.07.063

An integrated gel supported micro-fluidic system is reported, in which PCR products can be efficiently injected into a capillary electrophoresis device. The gel supported system is designed to provide greater stability to reagents during long periods... Read More about Development of a gel-to-gel electro-kinetic pinched injection method for an integrated micro-fluidic based DNA analyser.

Development of enzyme immobilized monolith micro-reactors integrated with microfluidic electrochemical cell for the evaluation of enzyme kinetics (2009)
Journal Article
Greenway, G., Haswell, S. J., & He, P. (2010). Development of enzyme immobilized monolith micro-reactors integrated with microfluidic electrochemical cell for the evaluation of enzyme kinetics. Microfluidics and Nanofluidics, 8(5), 565-573. https://doi.org/10.1007/s10404-009-0476-8

This paper describes a simple and efficient method for producing an on-chip enzyme immobilized monolith micro-reactor that integrates a microfluidic electrochemical cell for rapid characterization of enzymatic kinetics. The monolith was generated usi... Read More about Development of enzyme immobilized monolith micro-reactors integrated with microfluidic electrochemical cell for the evaluation of enzyme kinetics.

Electrogenerated chemiluminescence at droplet-modified electrodes: towards biphasic pKₐ measurement via proton-coupled electron transfer at liquid|liquid interfaces (2009)
Journal Article
Lledo-Fernandéz, C., Hatay, I., Ball, M. J., Greenway, G. M., & Wadhawan, J. (2009). Electrogenerated chemiluminescence at droplet-modified electrodes: towards biphasic pKₐ measurement via proton-coupled electron transfer at liquid|liquid interfaces. New journal of chemistry = Nouveau journal de chimie, 33(4), 749-759. https://doi.org/10.1039/b810844g

This preliminary work reports, for the first time, electrogenerated chemiluminescence (ECL) at droplet-modified electrodes. The system involves oxidation of aqueous tris(2,2′-bipyridyl)ruthenium(ii) ions at a glassy carbon electrode on which a random... Read More about Electrogenerated chemiluminescence at droplet-modified electrodes: towards biphasic pKₐ measurement via proton-coupled electron transfer at liquid|liquid interfaces.

The use of carrier RNA to enhance DNA extraction from microfluidic-based silica monoliths (2009)
Journal Article
Shaw, K. J., Thain, L., Dyer, C., Docker, P. T., Greenman, J., Greenway, G. M., & Haswell, S. J. (2009). The use of carrier RNA to enhance DNA extraction from microfluidic-based silica monoliths. Analytica Chimica Acta, 652(1-2), 231-233. https://doi.org/10.1016/j.aca.2009.03.038

DNA extraction was carried out on silica-based monoliths within a microfluidic device. Solid-phase DNA extraction methodology was applied in which the DNA binds to silica in the presence of a chaotropic salt, such as guanidine hydrochloride, and is e... Read More about The use of carrier RNA to enhance DNA extraction from microfluidic-based silica monoliths.

Development of a bi-functional silica monolith for electro-osmotic pumping and DNA clean-up/extraction using gel-supported reagents in a microfluidic device (2009)
Journal Article
Oakley, J. A., Shaw, K. J., Docker, P. T., Dyer, C. E., Greenman, J., Greenway, G. M., & Haswell, S. J. (2009). Development of a bi-functional silica monolith for electro-osmotic pumping and DNA clean-up/extraction using gel-supported reagents in a microfluidic device. Lab on a chip, 9(11), 1596-1600. https://doi.org/10.1039/b820553a

A silica monolith used to support both electro-osmotic pumping (EOP) and the extraction/elution of DNA coupled with gel-supported reagents is described. The benefits of the combined EOP extraction/elution system were illustrated by combining DNA extr... Read More about Development of a bi-functional silica monolith for electro-osmotic pumping and DNA clean-up/extraction using gel-supported reagents in a microfluidic device.

Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device (2009)
Journal Article
Shaw, K. J., Docker, P. T., Haswell, S. J., Joyce, D. A., Dyer, C. E., Greenman, J., & Greenway, G. M. (2009). Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device. Lab on a chip, 9(23), 3430-3432. https://doi.org/10.1039/b913309g

A novel DNA loading methodology is presented for performing DNA extraction on a microfluidic system. DNA in a chaotropic salt solution was manually loaded onto a silica monolith orthogonal to the subsequent flow of wash and elution solutions. DNA was... Read More about Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device.