All Outputs (20)

Environmental fluoxetine promotes skin cell proliferation and wound healing (2024)
Journal Article
Rodriguez-Barucg, Q., Garcia, A. A., Garcia-Merino, B., Akinmola, T., Okotie-Eboh, T., Francis, T., Bringas, E., Ortiz, I., Wade, M., Dowle, A., Joyce, D. A., Hardman, M. J., Wilkinson, H. N., & Beltran-Alvarez, P. (2024). Environmental fluoxetine promotes skin cell proliferation and wound healing. Environmental pollution, 362, Article 124952. https://doi.org/10.1016/j.envpol.2024.124952

This study investigates the effects of environmentally-relevant concentrations of fluoxetine (FLX, commercial name: Prozac) on wound healing. Pollution of water systems with pharmaceutical and personal care products, including antidepressants such as... Read More about Environmental fluoxetine promotes skin cell proliferation and wound healing.

Selective Depletion of Staphylococcus aureus Restores the Skin Microbiome and Accelerates Tissue Repair after Injury (2024)
Journal Article
Wilkinson, H. N., Stafford, A. R., Rudden, M., Rocha, N. D., Kidd, A. S., Iveson, S., Bell, A. L., Hart, J., Duarte, A., Frieling, J., Janssen, F., Röhrig, C., de Rooij, B., Ekhart, P. F., & Hardman, M. J. (2024). Selective Depletion of Staphylococcus aureus Restores the Skin Microbiome and Accelerates Tissue Repair after Injury. Journal of Investigative Dermatology, 144(8), 1865-1876. https://doi.org/10.1016/j.jid.2024.01.018

Our skin is home to a diverse community of commensal microorganisms integral to cutaneous function. However, microbial dysbiosis and barrier perturbation increase the risk of local and systemic infection. Staphylococcus aureus is a particularly probl... Read More about Selective Depletion of Staphylococcus aureus Restores the Skin Microbiome and Accelerates Tissue Repair after Injury.

The Host-Microbiota Axis in Chronic Wound Healing (2023)
Thesis
Stafford, A. R. The Host-Microbiota Axis in Chronic Wound Healing. (Thesis). Hull York Medical School. https://hull-repository.worktribe.com/output/4424929

Chronic, non-healing skin wounds represent a substantial area of unmet clinical need, leading to debilitating morbidity and mortality in affected individuals. Due to their high prevalence and recurrence, chronic wounds pose a significant economic bur... Read More about The Host-Microbiota Axis in Chronic Wound Healing.

The Skin Microbiome: Current Landscape and Future Opportunities (2023)
Journal Article
Smythe, P., & Wilkinson, H. N. (2023). The Skin Microbiome: Current Landscape and Future Opportunities. International Journal of Molecular Sciences, 24(4), Article 3950. https://doi.org/10.3390/ijms24043950

Our skin is the largest organ of the body, serving as an important barrier against the harsh extrinsic environment. Alongside preventing desiccation, chemical damage and hypothermia, this barrier protects the body from invading pathogens through a so... Read More about The Skin Microbiome: Current Landscape and Future Opportunities.

Epithelial arginase-1 is a key mediator of age-associated delayed healing in vaginal injury (2022)
Journal Article
Wilkinson, H. N., Reubinoff, B., Shveiky, D., Hardman, M. J., & Menachem-Zidon, O. B. (2022). Epithelial arginase-1 is a key mediator of age-associated delayed healing in vaginal injury. Frontiers in endocrinology, 13, Article 927224. https://doi.org/10.3389/fendo.2022.927224

Pelvic organ prolapse is a disorder that substantially affects the quality of life of millions of women worldwide. The greatest risk factors for prolapse are increased parity and older age, with the largest group requiring surgical intervention being... Read More about Epithelial arginase-1 is a key mediator of age-associated delayed healing in vaginal injury.

Cellular Senescence in Acute and Chronic Wound Repair (2022)
Journal Article
Wilkinson, H. N., & Hardman, M. J. (2022). Cellular Senescence in Acute and Chronic Wound Repair. Cold Spring Harbor Perspectives in Biology, 14(11), Article a041221. https://doi.org/10.1101/cshperspect.a041221

Cellular senescence, once thought an artifact of in vitro culture or passive outcome of aging, has emerged as fundamental to tissue development and function. The senescence mechanism importantly halts cell cycle progression to protect against tumor f... Read More about Cellular Senescence in Acute and Chronic Wound Repair.

Skin Aging in Long-Lived Naked Mole-Rats Is Accompanied by Increased Expression of Longevity-Associated and Tumor Suppressor Genes (2022)
Journal Article
Fatima, I., Chen, G., Botchkareva, N. V., Sharov, A. A., Thornton, D., Wilkinson, H. N., …Botchkarev, V. A. (in press). Skin Aging in Long-Lived Naked Mole-Rats Is Accompanied by Increased Expression of Longevity-Associated and Tumor Suppressor Genes. Journal of Investigative Dermatology, https://doi.org/10.1016/j.jid.2022.04.028

Naked mole-rats (NMRs) (Heterocephalus glaber) are long-lived mammals that possess a natural resistance to cancer and other age-related pathologies, maintaining a healthy life span >30 years. In this study, using immunohistochemical and RNA-sequencin... Read More about Skin Aging in Long-Lived Naked Mole-Rats Is Accompanied by Increased Expression of Longevity-Associated and Tumor Suppressor Genes.

Combined Metallomics/Transcriptomics Profiling Reveals a Major Role for Metals in Wound Repair (2021)
Journal Article
Wilkinson, H. N., Guinn, B. A., & Hardman, M. J. (2021). Combined Metallomics/Transcriptomics Profiling Reveals a Major Role for Metals in Wound Repair. Frontiers in Cell and Developmental Biology, 9, Article 788596. https://doi.org/10.3389/fcell.2021.788596

Endogenous metals are required for all life, orchestrating the action of diverse cellular processes that are crucial for tissue function. The dynamic wound healing response is underpinned by a plethora of such cellular behaviours, occurring in a time... Read More about Combined Metallomics/Transcriptomics Profiling Reveals a Major Role for Metals in Wound Repair.

Human ex vivo wound model and whole-mount staining approach to accurately evaluate skin repair (2021)
Journal Article
Wilkinson, H. N., Kidd, A. S., Roberts, E. R., & Hardman, M. J. (2021). Human ex vivo wound model and whole-mount staining approach to accurately evaluate skin repair. Journal of visualized experiments : JoVE, 168, Article e62326. https://doi.org/10.3791/62326

© 2021 JoVE Creative Commons. Chronic non-healing wounds, which primarily affect the elderly and diabetic, are a significant area of clinical unmet need. Unfortunately, current chronic wound treatments are inadequate, while available pre-clinical mod... Read More about Human ex vivo wound model and whole-mount staining approach to accurately evaluate skin repair.

Cellular benefits of single-use negative pressure wound therapy demonstrated in a novel ex vivo human skin wound model (2020)
Journal Article
Wilkinson, H. N., Longhorne, F. L., Roberts, E. R., Brownhill, V. R., & Hardman, M. J. (in press). Cellular benefits of single-use negative pressure wound therapy demonstrated in a novel ex vivo human skin wound model. Wound Repair and Regeneration, https://doi.org/10.1111/wrr.12888

Negative pressure wound therapy is a widely used treatment for chronic, nonhealing wounds. Surprisingly, few studies have systematically evaluated the cellular and molecular effects of negative pressure treatment on human skin. In addition, no study... Read More about Cellular benefits of single-use negative pressure wound therapy demonstrated in a novel ex vivo human skin wound model.

Wound healing: cellular mechanisms and pathological outcomes (2020)
Journal Article
Wilkinson, H. N., & Hardman, M. J. (2020). Wound healing: cellular mechanisms and pathological outcomes. Open Biology, 10(9), Article 200223. https://doi.org/10.1098/rsob.200223

Wound healing is a complex, dynamic process supported by a myriad of cellular events that must be tightly coordinated to efficiently repair damaged tissue. Derangement in wound-linked cellular behaviours, as occurs with diabetes and ageing, can lead... Read More about Wound healing: cellular mechanisms and pathological outcomes.

Wound healing: Cellular mechanisms and pathological outcomes (2020)
Journal Article
Wilkinson, H. N., & Hardman, M. J. (2020). Wound healing: Cellular mechanisms and pathological outcomes. Open Biology, 10(9), Article 200223. https://doi.org/10.1098/rsob.200223

Wound healing is a complex, dynamic process supported by a myriad of cellular events that must be tightly coordinated to efficiently repair damaged tissue. Derangement in wound-linked cellular behaviours, as occurs with diabetes and ageing, can lead... Read More about Wound healing: Cellular mechanisms and pathological outcomes.

Senescence in Wound Repair: Emerging Strategies to Target Chronic Healing Wounds (2020)
Journal Article
Wilkinson, H. N., & Hardman, M. J. (2020). Senescence in Wound Repair: Emerging Strategies to Target Chronic Healing Wounds. Frontiers in Cell and Developmental Biology, 8, Article 773. https://doi.org/10.3389/fcell.2020.00773

© Copyright © 2020 Wilkinson and Hardman. Cellular senescence is a fundamental stress response that restrains tumour formation. Yet, senescence cells are also present in non-cancerous states, accumulating exponentially with chronological age and cont... Read More about Senescence in Wound Repair: Emerging Strategies to Target Chronic Healing Wounds.

Pre-Clinical Assessment of Single-Use Negative Pressure Wound Therapy During In Vivo Porcine Wound Healing (2020)
Journal Article
Brownhill, V., Huddleston, E., Bell, A., Hart, J., Webster, I., Hardman, M., & Wilkinson, H. (2021). Pre-Clinical Assessment of Single-Use Negative Pressure Wound Therapy During In Vivo Porcine Wound Healing. Advances in wound care, 10(7), 345-356. https://doi.org/10.1089/wound.2020.1218

Objective: Traditional negative pressure wound therapy systems can be large and cumbersome, limiting patient mobility and adversely affecting quality of life. PICO™, a no canister single-use system offers a lightweight, portable alternative to tradit... Read More about Pre-Clinical Assessment of Single-Use Negative Pressure Wound Therapy During In Vivo Porcine Wound Healing.

Characterisation of a New Human Alveolar Macrophage-Like Cell Line (Daisy) (2019)
Journal Article
Sadofsky, L. R., Hayman, Y. A., Vance, J., Cervantes, J. L., Fraser, S. D., Wilkinson, H. N., Williamson, J. D., Hart, S. P., & Morice, A. H. (2019). Characterisation of a New Human Alveolar Macrophage-Like Cell Line (Daisy). Lung, 197(6), 687-698. https://doi.org/10.1007/s00408-019-00288-3

Purpose: There is currently no true macrophage cell line and in vitro experiments requiring these cells currently require mitogenic stimulation of a macrophage precursor cell line (THP-1) or ex vivo maturation of circulating primary monocytes. In thi... Read More about Characterisation of a New Human Alveolar Macrophage-Like Cell Line (Daisy).

Optimising platelet secretomes to deliver robust tissue-specific regeneration (2019)
Journal Article
Scully, D., Sfyri, P., Wilkinson, H. N., Acebes‐Huerta, A., Verpoorten, S., Muñoz‐Turrillas, M. C., Parnell, A., Patel, K., Hardman, M. J., Gutiérrez, L., & Matsakas, A. (2020). Optimising platelet secretomes to deliver robust tissue-specific regeneration. Journal of tissue engineering and regenerative medicine, 14(1), 82-98. https://doi.org/10.1002/term.2965

Promoting cell proliferation is the cornerstone of most tissue regeneration therapies. As platelet-based applications promote cell division and can be customised for tissue-specific efficacy, this makes them strong candidates for developing novel reg... Read More about Optimising platelet secretomes to deliver robust tissue-specific regeneration.

Tissue iron promotes wound repair via M2 macrophage polarisation and the chemokines CCL17 and CCL22 (2019)
Journal Article
Wilkinson, H. N., Roberts, E. R., Stafford, A. R., Banyard, K. L., Matteucci, P., Mace, K. A., & Hardman, M. J. (2019). Tissue iron promotes wound repair via M2 macrophage polarisation and the chemokines CCL17 and CCL22. American Journal of Pathology, 189(11), 2196-2208. https://doi.org/10.1016/j.ajpath.2019.07.015

Macrophages are important for effective iron recycling and erythropoiesis, but they also play a crucial role in wound healing, orchestrating tissue repair. Recently, we demonstrated a significant accumulation of iron in healing wounds and a requireme... Read More about Tissue iron promotes wound repair via M2 macrophage polarisation and the chemokines CCL17 and CCL22.

Reduced Iron in Diabetic Wounds: An Oxidative Stress-Dependent Role for STEAP3 in Extracellular Matrix Deposition and Remodeling (2019)
Journal Article
Wilkinson, H. N., Upson, S. E., Banyard, K. L., Knight, R., Mace, K. A., & Hardman, M. J. (2019). Reduced Iron in Diabetic Wounds: An Oxidative Stress-Dependent Role for STEAP3 in Extracellular Matrix Deposition and Remodeling. Journal of Investigative Dermatology, 139(11), 2368-2377.e7. https://doi.org/10.1016/j.jid.2019.05.014

Iron is crucial for maintaining normal bodily function with well-documented roles in erythropoiesis, hemostasis, and inflammation. Despite this, little is known about the temporal regulation of iron during wound healing, or how iron contributes to wo... Read More about Reduced Iron in Diabetic Wounds: An Oxidative Stress-Dependent Role for STEAP3 in Extracellular Matrix Deposition and Remodeling.

Elevated local senescence in diabetic wound healing is linked to pathological repair via CXCR2. (2019)
Journal Article
Wilkinson, H. N., Clowes, C., Banyard, K. L., Matteuci, P., Mace, K., & Hardman, M. J. (2019). Elevated local senescence in diabetic wound healing is linked to pathological repair via CXCR2. Journal of Investigative Dermatology, 139(5), 1171-1181.e6. https://doi.org/10.1016/j.jid.2019.01.005

© 2019 The Authors Cellular senescence can be broadly defined as a stable, but essentially irreversible, loss of proliferative capacity. Historically, senescence has been described as a negative outcome of advanced cellular age. It is now clear, howe... Read More about Elevated local senescence in diabetic wound healing is linked to pathological repair via CXCR2..

A novel silver bioactive glass elicits antimicrobial efficacy against Pseudomonas aeruginosa and Staphylococcus aureus in an ex vivo skin wound biofilm model (2018)
Journal Article
Wilkinson, H. N., Iveson, S., Catherall, P., & Hardman, M. J. (2018). A novel silver bioactive glass elicits antimicrobial efficacy against Pseudomonas aeruginosa and Staphylococcus aureus in an ex vivo skin wound biofilm model. Frontiers in Microbiology, 9(JUL), Article 1450. https://doi.org/10.3389/fmicb.2018.01450

Biofilm infection is now understood to be a potent contributor to the recalcitrant nature of chronic wounds. Bacterial biofilms evade the host immune response and show increased resistance to antibiotics. Along with improvements in antibiotic steward... Read More about A novel silver bioactive glass elicits antimicrobial efficacy against Pseudomonas aeruginosa and Staphylococcus aureus in an ex vivo skin wound biofilm model.