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Effects of antagonist of retinoid X receptor (UVI3003) on morphology and gene profile of Xenopus tropicalis embryos (2014)
Journal Article
Zhu, J., Shi, H., Zhu, P., Hu, L., Wu, L., Yang, Y., & Rotchell, J. M. (2014). Effects of antagonist of retinoid X receptor (UVI3003) on morphology and gene profile of Xenopus tropicalis embryos. Environmental Toxicology and Pharmacology, 38(1), 153-162. https://doi.org/10.1016/j.etap.2014.05.010

We exposed Xenopus tropicalis embryos to a selective antagonist of retinoid X receptor (UVI3003). UVI3003 induced multiple malformations at the concentrations of 200-1000. μg/L after 48. h exposure. The most prominent malformations affected brains, e... Read More about Effects of antagonist of retinoid X receptor (UVI3003) on morphology and gene profile of Xenopus tropicalis embryos.

Evidence of altered fertility in female roach (Rutilus rutilus) from the River Seine (France) (2014)
Journal Article
Gerbron, M., Geraudie, P., Fernandes, D., Rotchell, J. M., Porte, C., & Minier, C. (2014). Evidence of altered fertility in female roach (Rutilus rutilus) from the River Seine (France). Environmental pollution, 191, 58-62. https://doi.org/10.1016/j.envpol.2014.04.015

A large variety of anthropogenic chemicals present in the aquatic environment have been shown to be able to alter the endocrine system of exposed organisms, potentially impacting their reproductive function. The aim of this study was to assess the ef... Read More about Evidence of altered fertility in female roach (Rutilus rutilus) from the River Seine (France).

Advanced diagnostics applied to fish liver tumours: Relating pathology to underlying molecular aetiology (2013)
Journal Article
Lerebours, A., Bignell, J. P., Stentiford, G. D., Feist, S. W., Lyons, B. P., & Rotchell, J. M. (2013). Advanced diagnostics applied to fish liver tumours: Relating pathology to underlying molecular aetiology. Marine pollution bulletin, 72(1), 94-98. https://doi.org/10.1016/j.marpolbul.2013.04.016

Liver tumours in flatfish have been diagnosed using histopathology for decades in order to monitor the impacts of marine pollution in coastal and estuarine environments. This traditional method has been coupled with molecular analyses of tumours in t... Read More about Advanced diagnostics applied to fish liver tumours: Relating pathology to underlying molecular aetiology.

Intersex in scrobicularia plana: transcriptomic analysis reveals novel genes involved in endocrine disruption (2012)
Journal Article
Ciocan, C. M., Cubero-Leon, E., Peck, M. R., Langston, W. J., Pope, N., Minier, C., & Rotchell, J. M. (2012). Intersex in scrobicularia plana: transcriptomic analysis reveals novel genes involved in endocrine disruption. Environmental Science and Technology, 46(23), 12936-12942. https://doi.org/10.1021/es302948g

Intersex, the appearance of female characteristics in male gonads, has been identified in a wide range of aquatic species worldwide, yet the underpinning molecular etiology remains uncharacterised. The presence of intersex has been shown to be a wide... Read More about Intersex in scrobicularia plana: transcriptomic analysis reveals novel genes involved in endocrine disruption.

Combined effect of cadmium and estradiol on the endocrine system of roach (Rutilus rutilus): In vitro and in vivo approaches (2012)
Journal Article
Gerbron, M., Geraudie, P., Xuereb, B., Hill, E. M., Rotchell, J., & Minier, C. (2012). Combined effect of cadmium and estradiol on the endocrine system of roach (Rutilus rutilus): In vitro and in vivo approaches. Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 163(Supplement), S34-S34. https://doi.org/10.1016/j.cbpa.2012.05.099

Two CYP3A-like genes in the marine mussel Mytilus edulis: mRNA expression modulation following short-term exposure to endocrine disruptors (2012)
Journal Article
Cubero-Leon, E., Puinean, A. M., Labadie, P., Ciocan, C., Itoh, N., Kishida, M., Osada, M., Minier, C., Hill, E. M., & Rotchell, J. M. (2012). Two CYP3A-like genes in the marine mussel Mytilus edulis: mRNA expression modulation following short-term exposure to endocrine disruptors. Marine environmental research, 74, 32-39. https://doi.org/10.1016/j.marenvres.2011.11.012

Members of the vertebrate CYP3A subfamily are involved in the metabolism of steroids and a wide range of xenobiotics. In this study two CYP3A- like mRNAs have been isolated from the mussel (Mytilus edulis), and their seasonal expression profile and m... Read More about Two CYP3A-like genes in the marine mussel Mytilus edulis: mRNA expression modulation following short-term exposure to endocrine disruptors.

Metabolomic analysis of sex specific metabolites in gonads of the mussel, Mytilus edulis (2012)
Journal Article
Cubero-Leon, E., Minier, C., Hill, E. M., & Rotchell, J. (2012). Metabolomic analysis of sex specific metabolites in gonads of the mussel, Mytilus edulis. Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, 7(2), 212-219. https://doi.org/10.1016/j.cbd.2012.03.002

Marine mussels have been used as sentinel organisms to monitor exposure to a variety of chemical contaminants, including endocrine disrupting chemicals, in the aquatic environment. Although they are an important species for use in ecotoxicology inves... Read More about Metabolomic analysis of sex specific metabolites in gonads of the mussel, Mytilus edulis.

Transcriptional responses of cancer-related genes in turbot Scophthalmus maximus and mussels Mytilus edulis exposed to heavy fuel oil no. 6 and styrene (2012)
Journal Article
Ruiz, P., Orbea, A., Rotchell, J. M., & Cajaraville, M. P. (2012). Transcriptional responses of cancer-related genes in turbot Scophthalmus maximus and mussels Mytilus edulis exposed to heavy fuel oil no. 6 and styrene. Ecotoxicology, 21(3), 820-831. https://doi.org/10.1007/s10646-011-0843-6

Recent spills in European waters have released polycyclic aromatic hydrocarbons, important components of heavy fuel oil, and the hydrocarbon styrene. Heavy fuel oil and styrene are classified as potentially genotoxic and carcinogenic. Here we investi... Read More about Transcriptional responses of cancer-related genes in turbot Scophthalmus maximus and mussels Mytilus edulis exposed to heavy fuel oil no. 6 and styrene.

Reference gene selection for qPCR in mussel, Mytilus edulis, during gametogenesis and exogenous estrogen exposure (2012)
Journal Article
Cubero-Leon, E., Ciocan, C. M., Minier, C., & Rotchell, J. M. (2012). Reference gene selection for qPCR in mussel, Mytilus edulis, during gametogenesis and exogenous estrogen exposure. Environmental science and pollution research, 19(7), 2728-2733. https://doi.org/10.1007/s11356-012-0772-9

Purpose: The aim of this study is to develop a normalization method for real-time PCR data by analyzing the most stably expressed control genes in mussel (Mytilus edulis) reproductive tissue. Methods: To facilitate this, six candidate genes, includin... Read More about Reference gene selection for qPCR in mussel, Mytilus edulis, during gametogenesis and exogenous estrogen exposure.

Molecular toxicology of corals: A review (2011)
Journal Article
Rotchell, J. M., & Ostrander, G. K. (2011). Molecular toxicology of corals: A review. Journal of Toxicology and Environmental Health, Part B: Critical Reviews, 14(8), 571-592. https://doi.org/10.1080/10937404.2011.615112

Coral reefs worldwide have become increasingly affected by a phenomenon known as "coral bleaching," the loss of the symbiotic algae from the host corals. The underlying causes and mechanism(s) of coral bleaching are not well known, although several h... Read More about Molecular toxicology of corals: A review.

Analytical challenges and the development of biomarkers to measure and to monitor the effects of ocean acidification (2011)
Journal Article
Hardege, J., Rotchell, J., Terschak, J., & Greenway, G. (2011). Analytical challenges and the development of biomarkers to measure and to monitor the effects of ocean acidification. Trends in Analytical Chemistry, 30(8), 1320-1326. https://doi.org/10.1016/j.trac.2011.07.004

Changing ocean-carbonate chemistry caused by oceanic uptake of anthropogenic atmospheric carbon dioxide leads to the formation of carbonic acid, thus lowering the pH of the sea with predictions of a decrease from current levels at 8.15 to 7.82 by the... Read More about Analytical challenges and the development of biomarkers to measure and to monitor the effects of ocean acidification.

Identification of Reproduction-Specific genes associated with maturation and estrogen exposure in a marine bivalve Mytilus edulis (2011)
Journal Article
Ciocan, C. M., Cubero-Leon, E., Minier, C., & Rotchell, J. M. (2011). Identification of Reproduction-Specific genes associated with maturation and estrogen exposure in a marine bivalve Mytilus edulis. PLoS ONE, 6(7), e22326. https://doi.org/10.1371/journal.pone.0022326

Background: While it is established that vertebrate-like steroids, particularly estrogens (estradiol, estrone) and androgens (testosterone), are present in various tissues of molluscs, it is still unclear what role these play in reproductive endocrin... Read More about Identification of Reproduction-Specific genes associated with maturation and estrogen exposure in a marine bivalve Mytilus edulis.

A new in vitro screening bioassay for the ecotoxicological evaluation of the estrogenic responses of environmental chemicals using roach (Rutilus rutilus) liver explant culture (2010)
Journal Article
Gerbron, M., Geraudie, P., Rotchell, J., & Minier, C. (2010). A new in vitro screening bioassay for the ecotoxicological evaluation of the estrogenic responses of environmental chemicals using roach (Rutilus rutilus) liver explant culture. Environmental Toxicology, 25(5), 510-516. https://doi.org/10.1002/tox.20596

There is growing evidence that many chemicals released in the environment are able to disturb the normal endocrinology of organisms affecting the structure and function of their reproductive system. This has prompted the scientific community to devel... Read More about A new in vitro screening bioassay for the ecotoxicological evaluation of the estrogenic responses of environmental chemicals using roach (Rutilus rutilus) liver explant culture.

Effects of estrogen exposure in mussels, Mytilus edulis, at different stages of gametogenesis (2010)
Journal Article
Ciocan, C. M., Cubero-Leon, E., Puinean, A. M., Hill, E. M., Minier, C., Osada, M., Fenlon, K., & Rotchell, J. M. (2010). Effects of estrogen exposure in mussels, Mytilus edulis, at different stages of gametogenesis. Environmental pollution, 158(9), 2977-2984. https://doi.org/10.1016/j.envpol.2010.05.025

Mytilus edulis were exposed to 17β-estradiol (E2) and the synthetic estrogens ethinyl estradiol (EE2) and estradiol benzoate (EB) for 10 days. Two exposures were performed to determine their effect on vitellogenin (VTG) and estrogen receptor 2 (ER2)... Read More about Effects of estrogen exposure in mussels, Mytilus edulis, at different stages of gametogenesis.

Estrogens disrupt serotonin receptor and cyclooxygenase mRNA expression in the gonads of mussels (Mytilus edulis) (2010)
Journal Article
Cubero-Leon, E., Ciocan, C. M., Hill, E. M., Osada, M., Kishida, M., Itoh, N., Kondo, R., Minier, C., & Rotchell, J. M. (2010). Estrogens disrupt serotonin receptor and cyclooxygenase mRNA expression in the gonads of mussels (Mytilus edulis). Aquatic Toxicology, 98(2), 178-187. https://doi.org/10.1016/j.aquatox.2010.02.007

Estrogenic contaminants in the aquatic environment are associated with feminisation of male fish, however their effects on some invertebrate species, such as bivalve molluscs, have yet to be characterised. Gametogenesis represents a critical step in... Read More about Estrogens disrupt serotonin receptor and cyclooxygenase mRNA expression in the gonads of mussels (Mytilus edulis).

Anchoring novel molecular biomarker responses to traditional responses in fish exposed to environmental contamination (2009)
Journal Article
Nogueira, P., Pacheco, M., Lourdes Pereira, M., Mendo, S., & Rotchell, J. M. (2010). Anchoring novel molecular biomarker responses to traditional responses in fish exposed to environmental contamination. Environmental pollution, 158(5), 1783-1790. https://doi.org/10.1016/j.envpol.2009.11.007

The responses of Dicentrarchus labrax and Liza aurata to aquatic pollution were assessed in a contaminated coastal lagoon, using both traditional and novel biomarkers combined. DNA damage, assessed by comet assay, was higher in both fish species from... Read More about Anchoring novel molecular biomarker responses to traditional responses in fish exposed to environmental contamination.

Transcript profiling and DNA damage in the European eel (Anguilla anguilla L.) exposed to 7,12-dimethylbenz[a]anthracene (2009)
Journal Article
Nogueira, P., Lourenço, J., Rodriguez, E., Pacheco, M., Santos, C., Rotchell, J. M., & Mendo, S. (2009). Transcript profiling and DNA damage in the European eel (Anguilla anguilla L.) exposed to 7,12-dimethylbenz[a]anthracene. Aquatic Toxicology, 94(2), 123-130. https://doi.org/10.1016/j.aquatox.2009.06.007

The molecular responses induced during and after an acute exposure to 7,12-dimethylbenz[a]anthracene (DMBA) were analysed in liver, gill and blood cells of juvenile Anguilla anguilla with the aim of developing molecular biomarkers of environmental PA... Read More about Transcript profiling and DNA damage in the European eel (Anguilla anguilla L.) exposed to 7,12-dimethylbenz[a]anthracene.

Estrogenic activity of cadmium, copper and zinc in the yeast estrogen screen (2009)
Journal Article
Denier, X., Hill, E. M., Rotchell, J., & Minier, C. (2009). Estrogenic activity of cadmium, copper and zinc in the yeast estrogen screen. Toxicology in Vitro, 23(4), 569-573. https://doi.org/10.1016/j.tiv.2009.01.006

Heavy metals are increasingly studied due to their apparent ability to disrupt signaling pathways of living organisms including humans. Among various mechanisms of action, metals are suspected of exerting estrogenic activity in human and wildlife. In... Read More about Estrogenic activity of cadmium, copper and zinc in the yeast estrogen screen.

A novel population health approach: using fish retinoblastoma gene profiles as a surrogate for humans (2008)
Journal Article
Rotchell, J. M., du Corbier, F. A., Stentiford, G. D., Lyons, B. P., Liddle, A. R., & Ostrander, G. K. (2009). A novel population health approach: using fish retinoblastoma gene profiles as a surrogate for humans. Comparative Biochemistry and Physiology - Part C: Toxicology and Pharmacology, 149(2), 134-140. https://doi.org/10.1016/j.cbpc.2008.09.009

Retinoblastoma, a tumor suppressor gene, is frequently mutated in diverse types of human tumors. We have previously shown that two types of fish tumor, eye and liver, also possess mutant Rb genes. Our aim is to determine if the Rb allele status is li... Read More about A novel population health approach: using fish retinoblastoma gene profiles as a surrogate for humans.