Mr Graham Sellers
Long-PCR Study of Root-knot Nematodes
Project Description
Root-knot nematodes (Meloidogyne spp.) are endo-parasites that cause significant economic damage to almost all food crops. There are several quarantine, regulated and EPPO listed species and this group of pathogenic nematodes are routinely intercepted in trade. All life-stages are required to confirm the identity of a population with certainty using morphological techniques, however this is not always feasible. As a consequence. molecular methods are the principal tool for diagnosis of statutory interceptions.
As a follow on from the THYME project collaboration, this project aims to study the effectiveness of: long-PCR of root-knot nematode material, running these through a Flongle DNA sequencer, and utilising software developed at Hull university to classify to species. The project will report back on the utility of this method for diagnostics of crop pathogens.
| Project Acronym | PCRRKN |
|---|---|
| Status | Project Complete |
| Funder(s) | FERA |
| Value | £11,875.00 |
| Project Dates | Nov 1, 2022 - Mar 31, 2023 |
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