Martin Berger
Alterations in platelet alpha granule secretion and adhesion on collagen under flow in mice lacking the atypical Rho GTPase RhoBTB3
Berger, Martin; Riley, David R J; Lutz, Julia; Khalil, Jawad S; Aburima, Ahmed; Naseem, Khalid M; Rivero, Francisco
Authors
David R J Riley
Julia Lutz
Jawad S Khalil
Dr Ahmed Aburima A.Aburima@hull.ac.uk
Lecturer in Cardiovascular Science
Khalid M Naseem
Dr Francisco Rivero Crespo F.Rivero-Crespo@hull.ac.uk
Reader in Biomedical Science
Abstract
Typical Rho GTPases, like Rac1, Cdc42 and RhoA, act as molecular switches regulating various aspects of platelet cytoskeleton reorganization. The loss of these enzymes results in reduced platelet functionality. Atypical Rho GTPases of the RhoBTB subfamily are characterised 1 by divergent domain architecture. One family member, RhoBTB3, is expressed in platelets, but its function is unclear. In the present study we examined the role of RhoBTB3 in platelet function using a knockout mouse model. We found the platelet count, size, numbers of both alpha and dense granules and surface receptor profile in these mice were comparable to wild type mice. Deletion of Rhobtb3 had no effect on aggregation and dense granule secretion in response to a range of agonists including thrombin, collagen and ADP. By contrast, alpha granule secretion was increased in mice lacking RhoBTB3 in response to thrombin, CRP and U46619/ADP. Integrin activation and spreading on fibrinogen and collagen under static conditions were also unimpaired, however we observed reduced platelet accrual on collagen under flow conditions. These defects did not translate into alterations in tail bleeding time. We conclude that genetic deletion of Rhobtb3 leads to subtle alterations in alpha granule secretion and adhesion to collagen without significant effects on haemostasis in vivo.
Citation
Berger, M., Riley, D. R. J., Lutz, J., Khalil, J. S., Aburima, A., Naseem, K. M., & Rivero, F. (2019). Alterations in platelet alpha granule secretion and adhesion on collagen under flow in mice lacking the atypical Rho GTPase RhoBTB3. Cells, 8(2), 149. https://doi.org/10.3390/cells8020149
Journal Article Type | Article |
---|---|
Acceptance Date | Feb 7, 2019 |
Online Publication Date | Feb 11, 2019 |
Publication Date | Feb 11, 2019 |
Deposit Date | Feb 7, 2019 |
Publicly Available Date | Feb 8, 2019 |
Publisher | MDPI |
Peer Reviewed | Peer Reviewed |
Volume | 8 |
Issue | 2 |
Pages | 149 |
DOI | https://doi.org/10.3390/cells8020149 |
Keywords | Adhesion; Collagen; Platelets; Rho GTPases; RhoBTB3 |
Public URL | https://hull-repository.worktribe.com/output/1282572 |
Publisher URL | https://www.mdpi.com/2073-4409/8/2/149 |
Files
Article
(2.3 Mb)
PDF
Copyright Statement
© 2019 by the authors. Submitted for possible open access publication under the terms
and conditions of the Creative Commons Attribution (CC BY) license
(http://creativecommons.org/licenses/by/4.0/).
Article
(4.6 Mb)
PDF
Copyright Statement
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
You might also like
Thrombospondin-1 promotes hemostasis through modulation of cAMP signaling in blood platelets
(2020)
Journal Article
Platelet function following induced hypoglycaemia in type 2 diabetes
(2018)
Journal Article
Downloadable Citations
About Repository@Hull
Administrator e-mail: repository@hull.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search